文献类型：期刊文献

英文题名：Radiation-Induced Metabolomic Changes in Sterile Male Monochamus alternatus (Coleoptera: Cerambycidae)

作者：Qu, L. J.[1,2] Wang, L. J.[3] Zhang, Y. A.[2] Wang, Q. H.[2] Wang, Y. Z.[2] Zhao, T. H.[2] Cai, W. Z.[1]

第一作者：Qu, L. J.

通信作者：Cai, WZ[1]

机构：[1]China Agr Univ, Dept Entomol, Beijing 100193, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, State Forestry Adm, Key Lab Forest Protect, Beijing 100091, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2014

卷号：14

外文期刊名：JOURNAL OF INSECT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000349770200014)】；

基金：We greatly appreciate Prof. P. Jiang of the Forest Pest Control and Quarantine Bureau of Zhejiang Province of China for providing help with larvae collection and the irradiation of adult beetles. This work was supported by grants from the National Natural Science Foundation of China (30972378) and the National High Technology Research and Development Program of China (863 Program) (2012AA101503).

语种：英文

外文关键词：metabolite; vector insect; sterile insect technique; gas chromatography time-of-flight mass spectrometry

摘要：Radiation-induced sterile insect technique is a biologically based, environment-friendly method for the suppression or eradication of a number of insect pests. Although the basic mechanisms underlying the technology have been well studied, little is known about the cell responses in organisms. Characterization of the metabolic shift associated with radiation exposure in sterile insects would be helpful for understanding the detailed mechanism underlying this technique and promote its practical application. In this article, a metabolomic study was performed to characterize the global metabolic changes induced by radiation using untreated and 40 Gy Co-60 gamma-irradiated testes of Japanese pine sawyer, Monochamus alternatus Hope. Differential metabolites were detected and tentatively identified. Many key metabolites in glycolysis and the tricarboxylic acid cycle, as well as most fatty and amino acids, were elevated in irradiated male M. alternatus, presumably resulting from depression of glycolysis and the tricarboxylic acid cycle, each of which are important pathways for energy generation Adenosine Triphosphate (ATP) in insect spermatozoa. The findings in this article will contribute to our knowledge of the characteristic metabolic changes associated with irradiation sterility and understand the molecular mechanisms underlying radiation-induced sterile insect technique.