文献类型：期刊文献

中文题名：孝顺竹开花过程中DNA甲基化水平动态研究

英文题名：Features of DNA Methylation during the Flowering Process of Bambusa multiplex

作者：袁金玲[1] 郭广平[1] 岳晋军[1] 吴晓丽[1] 顾小平[1]

第一作者：袁金玲

机构：[1]中国林业科学研究院亚热带林业研究所

年份：2012

卷号：32

期号：1

起止页码：60-66

中文期刊名：西北植物学报

外文期刊名：Acta Botanica Boreali-Occidentalia Sinica

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

基金：浙江省重大科技专项重点项目(2009C12097);中国林业科学研究院亚热带林业研究所基本科研业务费(RISF6918)

语种：中文

中文关键词：孝顺竹;开花过程;DNA甲基化

外文关键词：Bambusa multiplex ; flowering process ; DNA methylation

分类号：Q789

摘要：以孝顺竹为材料,利用HPLC和MSAP技术在其未开花至开花的生长过程中进行甲基化水平的检测,分析竹子开花过程中基因组DNA甲基化的动态,以揭示DNA甲基化水平与竹子开花现象的相关性。结果显示:(1)孝顺竹基因组DNA甲基化率在不同的时间处于动态变化中,进入开花状态的孝顺竹植株其甲基化水平极显著低于开花竹丛中未开花植株和未开花竹丛;开花和未开花植株的总甲基化率分别为9.00%和12.42%,全甲基化率分别为5.06%和7.53%。(2)MSAP位点中有66.83%的位点在开花和未开花材料中甲基化状态保持一致,33.17%的位点在开花和未开花植株中发生甲基化变化:其中22.28%的位点在开花植株中发生完全的去甲基化,1.98%的谱带在开花植株中发生甲基化,8.91%的位点在开花和未开花材料中甲基化水平呈现上升或降低的趋势。研究表明,开花的孝顺竹同时发生甲基化和去甲基化,但发生去甲基化的概率明显大于发生甲基化的概率,最终导致其甲基化水平极显著降低。
To understand the DNA methylation features of bamboo during its flowering process, we em- ployed the techniques of HPLC （high performance liquid chromatography） and MSAP （methylation-sensi- tive amplified polymorphism） to detect the DNA methylation level of Bambusa multiplex （Gramineae, Bambusoideae） ,in a continuous period from its non-flowering to flowering stage. The＇results showed that DNA methylation rate of B. multiplex changed dynamically at different times. Furthermore,DNA methyla- tion rates of flowering plant were significantly lower than those of non-flowering plant and non-flowering clumps. Total DNA methylation rates of 9.00% and 12.42% were detected in flowering and non-flowering plant,respectively,with full-methylation of 5. 060% and 7.53%. Meanwhile, 66.83% MSAP sites were i- dentical both in flowering and non-flowering plants and 33.17% sites varied. Among those 33.17% varia- ble sites,22.28% were demethylation and 1.98% were methylation in flowering plants,and other 8.91% variable sites gave a variance tendency between flowering and non-flowering plants. Therefore,demethyla- tion and methylation happened simultaneously in flowering plants,with a much higher demethylation rate than that of methylation,thus exhibited a low methvlation rate in flowering nlants