文献类型：期刊文献

英文题名：Variations in microbial C-limitation and N-limitation dependent on stand age within soil aggregates of coastal saline Robinia plantations

作者：Xiong, Jing[1,3,4] Sun, Jianni[2] Wang, Xiaoyi[2] Shao, Xuexin[4] Xu, Haidong[2]

第一作者：Xiong, Jing

通信作者：Sun, JN[1];Xu, HD[1]

机构：[1]Jinggangshan Univ, Sch Life Sci, Jian 343009, Peoples R China;[2]Shandong Univ Aeronaut, Shandong Key Lab Eco Environm Sci Yellow River Del, Binzhou 256603, Peoples R China;[3]Jinggangshan Univ, Key Lab Jiangxi Prov Funct Biol & Pollut Control R, Jian 343009, Peoples R China;[4]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China

年份：2026

卷号：217

外文期刊名：APPLIED SOIL ECOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001633322400002)】；

基金：This research was financially supported by the Shandong Provincial Natural Science Foundation (No. ZR2023QC070) ; the National Natural Science Foundation of China (No. 42507427) ; the PhD Research Startup Foundation at Binzhou University (No. 2023Y15) ; and the College Stu-dent Innovation Training Program Plan (No. 202410449004) .

语种：英文

外文关键词：Ecoenzymatic stoichiometric theory; Microbial nutrient limitation; Soil aggregate; Afforestation; Stand age; The Yellow River Delta

摘要：The application of ecoenzymatic stoichiometric theory (EEST) models is a simple and effective method to estimate microbial nutrient limitation in terrestrial ecosystems. Ecological restoration of afforestation is a topic of broad interest, yet our understanding of microbial metabolic status in coastal restored soils, particularly at the aggregate scale, remains limited. Here, we investigated the activities of C-, N-, and P-acquiring enzymes within soil aggregates from different plantations of Robinia pseudoacacia (established in the spring of 1988, 2002, 2012, and 2018 using one-year-old seedlings in coastal undisturbed grasslands). Then, we applied the EEST models to examine how afforestation affects microbial nutrient limitation. We found that (1) soil C and N contents and their acquiring enzyme activities were significantly increased along the afforestation chronosequence, and were higher in macro-aggregates (> 0.25 mm) than in micro-aggregates (< 0.25 mm). (2) However, microbial metabolism in coastal soils was colimited by C and N, with a significant coupling relationship between C-limitation and N-limitation in macro-aggregates. Notably, afforestation significantly promoted the proportion of soil macro-aggregates, and alleviating their N limitation but intensifying C limitation, and the effect was more pronounced when the stand age exceeded 22 years. (3) Most plant traits significantly correlated with microbial nutrient limitation in coastal soils of R. pseudoacacia plantations. Therefore, plant trait modulation through ecological measures, such as improving the species composition and age structure of R. pseudoacacia plantations, might serve as an effective approach for managing microbial nutrient limitation and improving soil health in coastal saline-alkali ecosystems.