文献类型：期刊文献

英文题名：Effects of Low Temperature on Rhizosphere Phosphate-Mineralizing Microbial Populations in Constructed Wetlands

作者：Hao, Ting[1,2,3,4] Cui, Lijuan[1,2,3,4] Wang, Jinzhi[1,2,3,4] Lei, Yinru[1,2,3,4] Li, Wei[1,2,3,4] Wang, Rumiao[1,2,3,4] Wang, Shaokun[1,2,3,4] Li, Jing[1,2,3,4] Zhai, Xiajie[1,2,3,4] Zhang, Manyin[1,2,3,4] Zhao, Xinsheng[1,2,3,4]

第一作者：Hao, Ting

机构：[1] Institute of Wetland Research, Chinese Academy of Forestry, Beijing, 100091, China; [2] Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, Beijing, 100091, China; [3] Beijing Key Laboratory of Wetland Ecological Function and Restoration, Beijing, 100091, China; [4] Beijing Hanshiqiao National Wetland Ecosystem Research Station, Beijing, 101399, China

年份：2024

外文期刊名：SSRN

收录：EI(收录号：20240314869)

语种：英文

外文关键词：Alkalinity - Enzyme activity - Genes - Mineralogy - Organic carbon - Phosphatases - Phosphorus - Soils - Temperature - Wetlands

摘要：In constructed wetlands (CWs), the rhizosphere acts as a critical zone for phosphorus (P) mineralization and the sustenance of microbial activity. Despite this, the impact of low temperatures on phosphorus-related phosphatase activities and the composition and function of microbial communities during the winter is not fully comprehended. This study investigates the response of phosphatase activity and P-mineralizing microbial communities to decreased winter temperatures in two contrasting CWs. The research was conducted at two CWs, designated as LCW and HCW, to assess the effects of winter temperatures on phosphatase activity and P-mineralizing microbial communities. The study indicates that in January, characterized by the lowest temperatures, the enzymatic activities of alkaline phosphatase (ALP) and acid phosphatase (ACP) reached their nadir. Intriguingly, the enzyme activity levels in HCW exhibit a less pronounced decrease compared to those in LCW, a phenomenon attributed to the insulating effects of the ice layer. ALP activity in the rhizosphere consistently exceeded that of ACP and was significantly correlated with the expression levels of the phoD gene while ACP activity was significantly linked to the abundance of the phoC gene. Specifically, two distinct genera of phosphorus-mineralizing microorganisms have been identified: Sinorhizobium, has been the primary contributor to ALP secretion, while Stenotrophomonas, harboring the phoC gene, has been the key contributor to ACP secretion in winter. ALP activity was significantly affected by soil temperature and soluble sugar, whereas ACP activity was strongly negatively associated with pH, organic acid and total organic carbon (TOC). Additionally, low temperatures caused phoC-harboring bacteria to cluster more closely, which may help maintain their functional potential in P mineralization. These findings highlight the significance of regulating ice thickness in CWs to optimize P availability, especially during the colder seasons, by revealing the microbial community's adaptive mechanisms to environmental stress. ? 2024, The Authors. All rights reserved.