文献类型：期刊文献

英文题名：Characterization of Colletotrichum species associated with coffee berries in northern Thailand

作者：Prihastuti, H.[2,3,4] Cai, L.[1] Chen, H.[2] McKenzie, E. H. C.[5] Hyde, K. D.[2,3]

第一作者：Prihastuti, H.

通信作者：Cai, L[1]

机构：[1]Novozymes China, Beijing 100085, Peoples R China;[2]Chinese Acad Forestry, Res Inst Resource Insects, Int Fungal Res & Dev Ctr, Kunming 650224, Peoples R China;[3]Mae Fah Luang Univ, Sch Sci, Chiang Rai 57100, Thailand;[4]Brawijaya Univ, Fac Agr, Dept Biotechnol, Malang 65145, Indonesia;[5]Landcare Res, Auckland 1142, New Zealand

年份：2009

卷号：39

起止页码：89-109

外文期刊名：FUNGAL DIVERSITY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000273600400004)】；

基金：Double Degree Program under Biro Perencanaan dan Kerjasama Luar Negeri-Departemen Pendidikan Nasional Indonesia Fund is thanked for providing a masters scholarship. Part of this research was funded by research grants 51101010029 and 52101010002 awarded by Mae Fah Luang University. We are grateful to the Research Institute of Resource Insects for providing equipment and laboratory facilities for the molecular study. This research was also funded by AF Grant and the Grant for Essential Scientific Research of National Non-profit Institute (no. CAFYBB2007002) from Chinese Academy of Forestry.

语种：英文

外文关键词：anthracnose; disease; endophytes; epiphytes; multigene phylogeny; new species; pathogenicity

摘要：The identity of Colletotrichum species on Coffea (coffee) is not clearly understood. We report on Colletotrichum species associated with coffee berries in northern Thailand and compare them to species reported to cause coffee berry disease elsewhere. Morphological, cultural, biochemical and pathogenic characters, together with DNA sequence analyses resulted in the isolates clustering into three species: Colletotrichum asianum sp. nov., C. fructicola sp. nov. and C. siamense sp. nov. Phylogeny inferred from combined datasets of partial actin, beta-tubulin (tub2), calmodulin, glutamine synthetase, glyceraldehyde-3-phosphate dehydrogenase genes and the complete rDNA ITS1-5.8S-ITS2 regions revealed groupings that are congruent with morphological characters. Biochemical and DNA sequence data from multi-genes used here provided reliable data to differentiate between C. kahawae, C. gloeosporioides and the new Colletotrichum species.