文献类型：期刊文献

中文题名：基于CE-AFLP的5个美洲黑杨新品种指纹图谱分析

英文题名：Fingerprint Analysis of Five New Cultivars of Populus deltoides Based on CE-AFLP

作者：贾会霞[1] 胡建军[1] 卢孟柱[1]

第一作者：贾会霞

机构：[1]林木遗传育种国家重点实验室,中国林业科学研究院林业研究所,国家林业局林木培育重点实验室

年份：2013

卷号：26

期号：3

起止页码：281-286

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：林业行业公益类重大专项(201304103);"863"计划(2011AA100201);林木遗传育种国家重点实验室专项课题(CAFYBB2012041)

语种：中文

中文关键词：CE-AFLP;美洲黑杨;遗传多样性;指纹图谱

外文关键词：CE-AFLP; Populus deltoides ; fingerprint ; genetic diversity

分类号：S718.46

摘要：以5个美洲黑杨新品种(丹红杨、南杨、中林2025杨、中红杨和全红杨)为实验材料,利用CE-AFLP(毛细管电泳-AFLP)技术,采用EcoR I+3/Mse I+3和Pst I+2/Mse I+3引物组合构建指纹图谱,并进行遗传多样性分析。结果表明:从36对引物组合中筛选出12对扩增条带数目多、稳定且清晰的引物进行扩增,共获得条带数877条,多态性条带数315条,平均多态率为35.92%。采用UPGMA法进行聚类分析,相似系数为0.74～1.00;丹红杨与南杨之间的差异较大;在相似系数0.98时,中林2025杨与中红杨和全红杨分为两类,11条稳定特异的条带可以作为鉴别它们的依据;全红杨和中红杨之间未监测到稳定清晰的特异条带,初步认为2个品种在基因组水平上没有明显差异。
CE-AFLP（Capillary electrophoresis-amplified fragment length polymorphism）was used to construct the fingerprint and analyze the genetic diversity of five new cuhivars of Populus deltoides, including Populus deltoides CL. ‘Danhong＇ , P. deltoides CL. ‘Nan＇ , P. deltoides CL. ‘Zhonglin2025＇ , P. deltoides CL.‘Zhonghong＇ , and P. deltoides CL. ‘Quanhong＇. The primer combinations of EcoR I ＋ 3/Mse I ＋ 3 and Pst I ＋ 2/Mse I ＋ 3 were used in this study. The result indicated that 12 pairs of primers could be detected with many stable and clear amplified bands in all the 36 primer pairs. 877 bands were obtained, including 315 polymorphic bands, and the polymorphism frequency was 35.92%. UPGMA cluster analysis showed that the similarity coefficient ranged from 0.74 to 1.00. The difference between ‘Danhong＇ and ＇ Nan＇ was large. The similarity coefficient of ‘Zhonghong＇ and ‘Quanhong＇ with ‘Zhonglin2025＇ was 0.98.11 stable and clear specific bands could be used to identify these cultivars. There were not detected stable and clear specific bands between ‘Zhonghong＇ and ‘Quanhong＇. It sugges- ted that the variability of genomic between the two cuhivars was not dramatic.