文献类型：期刊文献

英文题名：Phenotype and transcriptome analysis identify the key genes controlling seed size and oil accumulation in oil-Camellia

作者：Liu, Linxiu[1,2,3] Sheng, Yu[1,3] Zhang, Yunbin[4] Xie, Xinru[1,3] Chen, Juanjuan[1,3] Wang, Jingfei[4] Pan, Huanhuan[1,3] Huang, Hu[1,3] Cao, Xun[1,3] Xu, Jing[1,3] Zhuo, Renying[1,3] Yao, Xiaohua[1,3]

第一作者：Liu, Linxiu

通信作者：Xu, J[1];Zhuo, RY[1];Yao, XH[1];Xu, J[2];Zhuo, RY[2];Yao, XH[2]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Breeding Zhejiang Prov, Hangzhou 311400, Zhejiang, Peoples R China;[2]Nanjing Forestry Univ, Fac Forestry, Nanjing 210000, Jiangsu, Peoples R China;[3]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[4]Mt Huangshan Forestry Res Inst, Huangshan 245099, Peoples R China

年份：2025

卷号：44

期号：4

外文期刊名：PLANT CELL REPORTS

收录：;Scopus(收录号：2-s2.0-105000550535);WOS:【SCI-EXPANDED(收录号:WOS:001449011200002)】；

基金：This research was supported by the National Key R & D Program of China (2019YFD1001602) and Fundamental Research Funds for the Central Non-profit Research Institution of Chinese Academy of Forestry (CAFYBB2017ZA004-1)

语种：英文

外文关键词：Oil;Camellia; F-1 population; Transcriptome; Seed size; Oil accumulation

摘要：Oil - Camellia is a major oil-producing tree, known for its high nutritional value and health benefits. Improving seed size and oil content is the key breeding objective, governed by intricate genetic networks. However, the molecular mechanism underlying these traits in oil-Camellia has been rarely reported. In this study, an F-1 population was developed from two parental genotypes, CL4 (hexaploid with high oil content and large seed size) and XG (tetraploid with low oil content and small seed size), which exhibited significant variation in yield-related traits. Ploidy analysis of progenies of F-1 population showed that most individuals were tetraploids and hexaploids, with a smaller number of diploids and octoploids present. Additionally, the analysis identified progeny individuals exhibiting both large seed and high oil content although no clear correlation with ploidy was observed. Comparative RNA sequencing (RNA seq) of developing seeds at four developmental stages revealed dynamic expression patterns, associated with seed size and oil content in the two parents. Co-expression regulatory network and differentially expressed genes of fatty acid biosynthesis pathway indicated that genes, such as Oleosin5 and ACCase alpha-subunit, displayed central roles in controlling seed size and oil content with notable expression peaks in S3 and S4. Additionally, the ABA signaling pathway, along with expansin proteins and transcription factors, showed co-expression with these genes, suggesting a regulatory pathway centered around Oleosin5 and ACCase alpha-subunit. This study identifies essential genes linked to oil seed yield traits, enhancing the understanding of oil-Camellia's molecular breeding targets.