详细信息
两步实验设计优化栅藻产油条件的工艺研究(英文)
A Two-step Statistical Optimization of Cultivation Conditions for Producing Lipid by Scenedesmus obliquus
文献类型:期刊文献
中文题名:两步实验设计优化栅藻产油条件的工艺研究(英文)
英文题名:A Two-step Statistical Optimization of Cultivation Conditions for Producing Lipid by Scenedesmus obliquus
作者:杨静[1,2,3,4] 蒋剑春[1,2,3,4] 张宁[1,2,3,4] 卫民[1,2,3,4]
第一作者:杨静
机构:[1]中国林业科学研究院林产化学工业研究所;[2]生物质化学利用国家工程实验室;[3]国家林业局林产化学工程重点开放性实验室;[4]江苏省生物质能源与材料重点实验室
年份:2011
卷号:31
期号:6
起止页码:7-10
中文期刊名:林产化学与工业
外文期刊名:Chemistry and Industry of Forest Products
收录:CSTPCD;;Scopus;北大核心:【北大核心2008】;CSCD:【CSCD_E2011_2012】;
基金:林业公益性行业专项(201004001)
语种:中文
中文关键词:栅藻;部分析因设计;正交试验;油脂产率
外文关键词:Scenedesmus obliquus ; fractional factorial design; orthogonal design; lipid productivity
分类号:TQ35;TQ517
摘要:提出了一种简单、快速优化栅藻(Scenedesmus obliquus)产油条件的两步实验设计方法。首先,利用部分析因设计方法从6个因素(光照强度,温度,pH值,接种量,葡萄糖浓度,氮源浓度)中筛选出显著因素。再利用正交试验进一步确定出最优条件。结果表明:氮源浓度和温度是影响栅藻产油的显著因素。适合培养栅藻产油的最优条件为:氮源6.0 mmol/L,温度25℃和光照80μmol/(m2.s),最高油脂产率达到75.46 mg/(L.d)。
A fast and easy two-step statistical optimization of cuhivation condition for producing lipid by Scenedesmus obliquus was described. At first, a fractional factorial design (FFD) was used to screen significant factors from six factors ( illumination, temperature, pH value, inoculation, glucose concentration and nitrogen source concentration). Then an orthogonal experiment was executed to further determine optimal conditions. According to the results, nitrogen source concentration and temperature were selected as significant factors. The optimal conditions occurred at nitrogen source concentration 6.0 mmol/L, temperature 25 ℃ and illumination 80 umol/( m2. s), with the highest lipid productivity of 75.46 mg/(L. d).
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