文献类型：期刊文献

中文题名：酸雨对木芙蓉幼苗光合作用及抗氧化酶活性的影响

英文题名：EFFECTS OF ACID RAIN ON PHOTOSYNTHESIS AND ANTIOXIDANT ENZYME ACTIVITY OF H. mitabilis L. SEEDLINGS

作者：王应军[1] 邓仕槐[1] 姜静[1] 尚鹤[2] 林波[2,3] 孙亚琴[1] 胡晓梅[1]

第一作者：王应军

机构：[1]四川农业大学资源环境学院农业环境工程省重点实验室;[2]中国林业科学研究院森林生态环境与保护研究所;[3]北京林业大学林学院

年份：2011

卷号：25

期号：3

起止页码：588-593

中文期刊名：核农学报

外文期刊名：Journal of Nuclear Agricultural Sciences

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家"十一五"计划攻关项目(2006BAD03A0104-2);四川省教育厅重点项目(10ZA059)

语种：中文

中文关键词：木芙蓉;模拟酸雨;光合作用;抗氧化酶活性

外文关键词：H.mitabilis L.; simulated acid rain; photosynthesis; antioxidant enzyme activity

分类号：S565.4

摘要：以pH 5.6为对照,采用pH 4.0、pH 3.0、pH 2.0强度的酸雨对2年生木芙蓉进行人工模拟胁迫,研究酸雨胁迫对木芙蓉叶片可见伤害、质膜透性(Membrane Permeability,MP)、叶绿素(Chlorophyll,Chl)含量、抗氧化酶系统及气体交换参数的影响。研究结果表明,pH 2.0和pH 3.0的酸雨处理使叶片出现可见伤害;木芙蓉细胞膜透性在酸雨胁迫处理下均明显增加,并与pH值呈极显著负相关(r=-0.961**);超氧化物歧化酶(Superoxide Dismutase,SOD)活性在pH 3.0处理下最高(216.80U.g-1.FW),过氧化氢酶(Catalase,CAT)和过氧化物酶(Peroxidase,POD)活性在pH 2.0处理下最强(分别为48.75mg.min-1.g-1.FW和77.69U.min-1.g-1.FW);叶绿素a、叶绿素b、总叶绿素、叶绿素a/b、净光合速率(Net Photosynthetic Rate,Pn)、气孔导度(Stomatal Conductivity,Gs)和蒸腾速率(TranspirationRate,Tr)均随pH值的降低而降低,胞间CO2浓度(Intercellular CO2 Concentration,Ci)呈先降后升的趋势,而水分利用效率(Water Use Efficiency,WUE)先升高后降低。模拟酸雨对木芙蓉的可见伤害阈值小于pH 2.0,对木芙蓉生理活动影响阈值大致在pH 2.0～3.0,表明木芙蓉对酸雨具有较强的耐受性。本研究为木芙蓉栽培管理、抗性筛选,以及为南方城市绿化树种的选择提供理论依据。
A pot experiment was conducted to study the effects of simulated acid rain on foliar damage,chlorophyll （Chl） content,membrane permeability（MP）,antioxidant enzyme activity and gas exchange parameters in leaves of 2 -year H.mitabilis seedlings.Four gradients of simulated acid rain solutions of pH 5.6（CK）,4.0,3.0 and 2.0 were used.Results showed that the leaves treated by pH 2.0 and 3.0 showed visible injury spots to various extent.The membrane permeability（MP） increased significantly after acid rain stress with an extremely significant negative correlation to pH value（r =-0.961＊＊）.The maximum catalase（CAT） activity and peroxidase（POD） activity were observed at pH 2.0（48.75mg · min-1 · g-1 · FW and 77.69U · min-1 · g-1 · FW,respectively）,while that of superoxide dismutase（SOD） activity were observed at the pH 3.0（216.80U·g-1 ·FW）.Chlorophyll a（Chl a）, chlorophyll b（Chl b）,total chlorophyll content,Chl a/b,net photosynthetic rate（Pn）,stomatal conductivity（Gs） and transpiration rate（Tr） decreased with decreaseing pH value of pH,and intercellular CO2concentration（Ci） showed a trend of decreasing first and then increasing,while water use efficiency（WUE） was opposite.The visible damage threshold of H.mitabilis by simulated acid rain was less than pH 2.0,and the threshold on the physiological activity was among pH 2.0 to 3.0,which indicated that H.mitabilis had higher tolerance to acid rain stress.This study could provide theoretical reference for cultivation management and resistance screening of H.mitabilis,and offer basic theory to choose the greening tree species in Southern cities.