文献类型：期刊文献

英文题名：Divergence of a genomic island leads to the evolution of melanization in a halophyte root fungus

作者：Yuan, Zhilin[1,2] Druzhinina, Irina S.[3] Gibbons, John G.[4] Zhong, Zhenhui[5,6] Van de Peer, Yves[7] Rodriguez, Russell J.[8] Liu, Zhongjian[9] Wang, Xinyu[2] Wei, Huanshen[1,2] Wu, Qi[10] Wang, Jieyu[11] Shi, Guohui[10] Cai, Feng[3] Peng, Long[1,2] Martin, Francis M.[12,13]

第一作者：袁志林

通信作者：Yuan, ZL[1];Yuan, ZL[2];Martin, FM[3];Martin, FM[4]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou, Peoples R China;[3]Nanjing Agr Univ, Coll Resources & Environm Sci, Fungal Genom Lab FungiG, Nanjing, Peoples R China;[4]Univ Massachusetts, Dept Food Sci, Amherst, MA 01003 USA;[5]Fujian Agr & Forestry Univ, State Key Lab Ecol Pest Control Fujian & Taiwan C, Coll Plant Protect, Fuzhou, Peoples R China;[6]Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA USA;[7]Univ Ghent, Dept Plant Biotechnol & Bioinformat, Ghent, Belgium;[8]Univ Washington, Adapt Symbiot Technol, Seattle, WA 98195 USA;[9]Fujian Agr & Forestry Univ, Key Lab Natl Forestry & Grassland Adm Orchid Cons, Coll Landscape Architecture, Fuzhou, Peoples R China;[10]Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Beijing, Peoples R China;[11]Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou, Peoples R China;[12]Beijing Forestry Univ, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Beijing, Peoples R China;[13]Univ Lorraine, INRAE, UMR Interact Arbres Microorganismes, Ctr INRAE Grand Est Nancy, Champenoux, France

年份：2021

卷号：15

期号：12

起止页码：3468-3479

外文期刊名：ISME JOURNAL

收录：;Scopus(收录号：2-s2.0-85107432087);WOS:【SCI-EXPANDED(收录号:WOS:000659428200001)】；

基金：This work was financially supported by the Fundamental Research Funds for the Central Non-profit Research of the Chinese Academy of Forestry (CAFYBB2020ZY002-1) and the National Natural Science Foundation of China (No. 31722014). Research in FMM's laboratory is supported by the Laboratory of Excellence ARBRE (ANR-11-LABX-0002-01) and the Beijing Advanced Innovation Center for Tree Breeding by Molecular Design. We extend our sincere gratitude to Dr. Fabien Halkett (INRAE, Universite de Lorraine), Dr. Fengyan Bai (State Key Laboratory of Mycology, Chinese Academy of Sciences), and Dr. Shu Zhao (University of Massachusetts) for many useful comments.

语种：英文

摘要：Understanding how organisms adapt to extreme living conditions is central to evolutionary biology. Dark septate endophytes (DSEs) constitute an important component of the root mycobiome and they are often able to alleviate host abiotic stresses. Here, we investigated the molecular mechanisms underlying the beneficial association between the DSE Laburnicola rhizohalophila and its host, the native halophyte Suaeda salsa, using population genomics. Based on genome-wide Fst (pairwise fixation index) and Vst analyses, which compared the variance in allele frequencies of single-nucleotide polymorphisms (SNPs) and copy number variants (CNVs), respectively, we found a high level of genetic differentiation between two populations. CNV patterns revealed population-specific expansions and contractions. Interestingly, we identified a similar to 20 kbp genomic island of high divergence with a strong sign of positive selection. This region contains a melanin-biosynthetic polyketide synthase gene cluster linked to six additional genes likely involved in biosynthesis, membrane trafficking, regulation, and localization of melanin. Differences in growth yield and melanin biosynthesis between the two populations grown under 2% NaCl stress suggested that this genomic island contributes to the observed differences in melanin accumulation. Our findings provide a better understanding of the genetic and evolutionary mechanisms underlying the adaptation to saline conditions of the L. rhizohalophila-S. salsa symbiosis.