文献类型：期刊文献

英文题名：Functional Characterization of a Dna-Damage Repair/Tolerance 100 (Drt100) Gene in Sedum Alfredii Hance for Genome Stability Maintenance and Cd Hypertolerance

作者：Liu, Mingying[1] He, Xuelian[2] Mu, Ju[1] Zhuo, Renying[3,4] Zhang, Dayi[5,6]

第一作者：Liu, Mingying

机构：[1] School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, 310053, China; [2] Fungal Research Center, Shaanxi Provincial Institute of Microbiology, Shaanxi, Xi’an, 710043, China; [3] State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, 100091, China; [4] The Research Institute of Subtropical of Forestry, Chinese Academy of Forestry, Hangzhou, 311400, China; [5] Key Laboratory of Groundwater Resources and Environment, Jilin University, Ministry of Education, Changchun, 130021, China; [6] College of New Energy and Environment, Jilin University, Changchun, 130021, China

年份：2022

外文期刊名：SSRN

收录：EI(收录号：20220408853)

语种：英文

外文关键词：Antennas - Bioremediation - Cadmium - DNA - Genetic engineering - Plants (botany) - Repair - Soil pollution

摘要：Cd contamination is a world-wild concern for its toxicity and accumulation in food chain. Sedum alfredii Hance (Crassulaceae) is a zinc-cadmium (Zn/Cd) hyperaccumulator native to China and widely applied for the phytoremediation of Zn and Cd contaminated soils. Although numerous studies have explored the uptake, translocation and storage of Cd in S. alfredii Hance, there is still lack of comprehensive studies on genes and underlying mechanisms of genome stability maintenance under Cd stress. In the present study, a gene resembling DNA-damage repair/toleration 100 (DRT100) was Cd inducible and designated as SaDRT100. Heterologous expression of SaDRT100 gene in yeasts and Arabidopsis thaliana enhanced Cd tolerance capability. Under Cd stress, transgenic Arabidopsis with SaDRT100 gene exhibited lower levels of reactive oxygen species (ROS), fewer Cd uptake in roots and less Cd-induced DNA damage. Evidenced by the subcellular location in cellular nucleus and expression in aerial parts, we suggested the involvement of SaDRT100 in combating Cd-induced DNA damage. Our findings firstly uncovered the roles of SaDRT100 gene in Cd hypertolerance and genome stability maintenance in S. alfredii Hance. The potential functions of DNA protection make SaDRT100 gene a candidate in genetic engineering for phytoremediation at multi-component contaminated sites. ? 2022, The Authors. All rights reserved.