文献类型：期刊文献

英文题名：Synonymous mutation in Growth Regulating Factor 15 of miR396a target sites enhances photosynthetic efficiency and heat tolerance in poplar

作者：Zhao, Yiyang[1,2,3] Xie, Jianbo[1,2,3] Wang, Sha[1,2,3] Xu, Weijie[1,2,3] Chen, Sisi[1,2,3] Song, Xueqin[4] Lu, Mengzhu[4,5] El-Kassaby, Yousry A.[6] Zhang, Deqiang[1,2,3]

第一作者：Zhao, Yiyang

通信作者：Zhang, DQ[1];Zhang, DQ[2];Zhang, DQ[3]

机构：[1]Beijing Forestry Univ, Beijing Adv Innovat Ctr Tree Breeding Mol Design, 35 Qinghua East Rd, Beijing 100083, Peoples R China;[2]Beijing Forestry Univ, Coll Biol Sci & Technol, Natl Engn Lab Tree Breeding, Beijing 100083, Peoples R China;[3]Beijing Forestry Univ, Coll Biol Sci & Technol, Key Lab Genet & Breeding Forest Trees & Ornamenta, Minist Educ, Beijing 100083, Peoples R China;[4]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[5]Zhejiang Agr & Forestry Univ, Hangzhou 311300, Peoples R China;[6]Univ British Columbia, Fac Forestry, Forest Sci Ctr, Dept Forest & Conservat Sci, Vancouver, BC V6T 1Z4, Canada

年份：2021

卷号：72

期号：12

起止页码：4502-4519

外文期刊名：JOURNAL OF EXPERIMENTAL BOTANY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000661482800022)】；

基金：The study was supported by the Project of National Natural Science Foundation of China (Nos 31872671 and 31500550) and the 111 Project (No. B20050).The funding body had no involvement in designing the study or collecting, analysing, or interpretating data, or in writing the manuscript.

语种：英文

外文关键词：Gene regulatory network (GRN); growth regulating factor (GRF); heat stress (HS); miR396a; photosynthesis; poplar; Populus

摘要：Heat stress damages plant tissues and induces multiple adaptive responses. Complex and spatiotemporally specific interactions among transcription factors (TFs), microRNAs (miRNAs), and their targets play crucial roles in regulating stress responses. To explore these interactions and to identify regulatory networks in perennial woody plants subjected to heat stress, we integrated time-course RNA-seq, small RNA-seq, degradome sequencing, weighted gene correlation network analysis, and multi-gene association approaches in poplar. Results from Populus trichocarpa enabled us to construct a three-layer, highly interwoven regulatory network involving 15 TFs, 45 miRNAs, and 77 photosynthetic genes. Candidate gene association studies in a population of P tomentosa identified 114 significant associations and 696 epistatic SNP-SNP pairs that were linked to 29 photosynthetic and growth traits (P<0.0001, q<0.05). We also identified miR396a and its target, Growth-Regulating Factor 15 (GRF15) as an important regulatory module in the heat-stress response. Transgenic plants of hybrid poplar (P. alba x P. glandulosa) overexpressing a GRF15 mRNA lacking the miR396a target sites exhibited enhanced heat tolerance and photosynthetic efficiency compared to wild-type plants. Together, our observations demonstrate that GRF15 plays a crucial role in responding to heat stress, and they highlight the power of this new, multifaceted approach for identifying regulatory nodes in plants.