文献类型：期刊文献

英文题名：CRISPR/Cas9 mutated p-coumaroyl shikimate 3'-hydroxylase 3 gene in Populus tomentosa reveals lignin functioning on supporting tree upright

作者：Zhang, Sufang[1] Wang, Bo[2] Li, Qian[1] Hui, Wenkai[1] Yang, Linjie[3] Wang, Zhihua[1] Zhang, Wenjuan[1] Yue, Fengxia[3] Liu, Nian[1] Li, Huiling[1] Lu, Fachuang[3,4,5] Zhang, Kewei[6] Zeng, Qingyin[7] Wu, Ai-Min[1]

第一作者：Zhang, Sufang

通信作者：Wu, AM[1];Zeng, QY[2]

机构：[1]South China Agr Univ, Coll Forestry & Landscape Architectures, Guangdong Key Lab Innovat Dev & Utilizat Forest Pl, Guangzhou 510642, Peoples R China;[2]South China Agr Univ, Coll Agr, Guangzhou 510642, Peoples R China;[3]South China Univ Technol, Sch Light Ind & Engn, State Key Lab Pulp & Paper Engn, Guangzhou 510640, Peoples R China;[4]Univ Wisconsin, Wisconsin Energy Inst, Dept Biochem, Madison, WI 53726 USA;[5]Univ Wisconsin, Wisconsin Energy Inst, Great Lakes Bioenergy Res Ctr, Madison, WI 53726 USA;[6]Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Zhejiang, Peoples R China;[7]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2023

卷号：253

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001081363300001)】；

基金：We thank Weihui Li and Xianhai Zhao from South China Agricultural University for excellent technical assistance and discussions. We also thank Tsuyoshi Nakagawa from Shimane University for supplying the pGWB101 vector. This work was supported by National Key Researc-hand Development Program of China (2022YFD2200102) , National Natural Science Foundation of China (Grant Nos. 31870653 and 31670670) , Key Project of Guangzhou Science and Technology Plan (201904020014) , Natural Science Foundation of Guangdong Province, China (2020A1515011009) .

语种：英文

外文关键词：c3 'h3 mutant; Secondary metablism; Lignin; Dwarf; Flavonoid; Populus tomentosa

摘要：The lignin plays one of the most important roles in plant secondary metabolism. However, it is still unclear how lignin can contribute to the impressive height of wood growth. In this study, C3'H, a rate-limiting enzyme of the lignin pathway, was used as the target gene. C3'H3 was knocked out by CRISPR/Cas9 in Populus tomentosa. Compared with wild-type popular trees, c3'h3 mutants exhibited dwarf phenotypes, collapsed xylem vessels, weakened phloem thickening, decreased hydraulic conductivity and photosynthetic efficiency, and reduced auxin content, except for reduced total lignin content and significantly increased H-subunit lignin. In the c3'h3 mutant, the flavonoid biosynthesis genes CHS, CHI, F3H, DFR, ANR, and LAR were upregulated, and flavonoid metabolite accumulations were detected, indicating that decreasing the lignin biosynthesis pathway enhanced flavonoid metabolic flux. Furthermore, flavonoid metabolites, such as naringenin and hesperetin, were largely increased, while higher hesperetin content suppressed plant cell division. Thus, studying the c3'h3 mutant allows us to deduce that lignin deficiency suppresses tree growth and leads to the dwarf phenotype due to collapsed xylem and thickened phloem, limiting material exchanges and transport.