文献类型：期刊文献

英文题名：Identification of the Genes Involved in Anthocyanin Biosynthesis and Accumulation in Taxus chinensis

作者：Zhang, Lisha[1,2] Sun, Xiaomei[1,2] Wilson, Iain W.[3] Shao, Fenjuan[1,2] Qiu, Deyou[1,2]

第一作者：Zhang, Lisha

通信作者：Shao, FJ[1];Shao, FJ[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Natl Forestry & Grassland Adm, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat, Natl Forestry & Grassland Adm, Beijing 100091, Peoples R China;[3]CSIRO Agr & Food, POB 1600, Canberra, ACT 2601, Australia

年份：2019

卷号：10

期号：12

外文期刊名：GENES

收录：;Scopus(收录号：2-s2.0-85075977671);WOS:【SCI-EXPANDED(收录号:WOS:000507342400036)】；

基金：The work was supported by the National Key Research and Development Program of China (grant number 2017YFD0600205) and the Natural Science Foundation of China (grant numbers 31700584 and 31670676).

语种：英文

外文关键词：anthocyanins; Taxus chinensis; gymnosperm

摘要：Taxus chinensis is a precious woody species with significant economic value. Anthocyanin as flavonoid derivatives plays a crucial role in plant biology and human health. However, the genes involved in anthocyanin biosynthesis have not been identified in T. chinensis. In this study, twenty-five genes involved in anthocyanin biosynthesis were identified, including chalcone synthase, chalcone isomerase, flavanone 3-hydroxylase, anthocyanidin synthase, flavonoid 3'-hydroxylase, flavonoid 3',5'-hydroxylase, dihydroflavonol 4-reductase, anthocyanidin reductase, and leucoanthocyanidin reductase. The conserved domains and phylogenetic relationships of these genes were characterized. The expression levels of these genes in different tissues and different ages of xylem were investigated. Additionally, the anthocyanin accumulation in xylem of different ages of T. chinensis was measured. The results showed the anthocyanin accumulation was correlated with the expression levels of dihydroflavonol 4-reductase, anthocyanidin synthase, flavonoid 3'-hydroxylase, and flavonoid 3',5'-hydroxylase. Our results provide a basis for studying the regulation of the biosynthetic pathway for anthocyanins and wood color formation in T. chinensis.