文献类型：期刊文献

英文题名：Transcriptional and temporal response of Populus stems to gravi-stimulation

作者：Zinkgraf, Matthew[1,2,5] Gerttula, Suzanne[1,2] Zhao, Shutang[3] Filkov, Vladimir[2] Groover, Andrew[1,4]

第一作者：Zinkgraf, Matthew

通信作者：Groover, A[1];Groover, A[2]

机构：[1]US Forest Serv, USDA, Pacific Southwest Res Stn, 1731 Res Pk Dr, Davis, CA 95618 USA;[2]Univ Calif Davis, Dept Comp Sci, One Shields Ave, Davis, CA 95618 USA;[3]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[4]Univ Calif Davis, Dept Plant Biol, One Shields Ave, Davis, CA 95618 USA;[5]Western Washington Univ, Dept Biol, 516 High St, Bellingham, WA 98225 USA

年份：2018

卷号：60

期号：7

起止页码：578-590

外文期刊名：JOURNAL OF INTEGRATIVE PLANT BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000437122800005)】；

基金：We thank Courtney Canning and Annie Mix at the Institute of Forest Genetics for their help in maintaining the greenhouse experiment. This work was supported by grant 2015-67013-22891 USDA AFRI to A.G. and V.F.. M.Z. is supported by NSF PGRP Fellowship grant IOS-1402064. This work used the Vincent J. Coates Genomics Sequencing Laboratory at UC Berkeley, supported by NIH S10 Instrumentation Grants S10RR029668 and S10RR027303.

语种：英文

摘要：Plants modify development in response to external stimuli, to produce new growth that is appropriate for environmental conditions. For example, gravi-stimulation of leaning branches in angiosperm trees results in modifications of wood development, to produce tension wood that pulls leaning stems upright. Here, we use gravi-stimulation and tension wood response to dissect the temporal changes in gene expression underlying wood formation in Populus stems. Using time-series analysis of seven time points over a 14-d experiment, we identified 8,919 genes that were differentially expressed between tension wood (upper) and opposite wood (lower) sides of leaning stems. Clustering of differentially expressed genes showed four major transcriptional responses, including gene clusters whose transcript levels were associated with two types of tissue-specific impulse responses that peaked at about 24-48h, and gene clusters with sustained changes in transcript levels that persisted until the end of the 14-d experiment. Functional enrichment analysis of those clusters suggests they reflect temporal changes in pathways associated with hormone regulation, protein localization, cell wall biosynthesis and epigenetic processes. Time-series analysis of gene expression is an underutilized approach for dissecting complex developmental responses in plants, and can reveal gene clusters and mechanisms influencing development.