文献类型：期刊文献

英文题名：Melatonin enhances nitrogen metabolism and haustorium development in hemiparasite Santalum album Linn.

作者：Meng, Sen[1] Wang, Xiaoling[2] Bian, Zhan[1] Li, Zhenshuang[1] Yang, Fucheng[1] Wang, Shengkun[1] Yoder, John, I[3] Lu, Junkun[1]

第一作者：孟森

通信作者：Lu, JK[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, State Key Lab Tree Genet & Breeding, Guangzhou 510520, Guangdong, Peoples R China;[2]Jiangxi Acad Sci, Inst Biol Resources, Nanchang 30096, Jiangxi, Peoples R China;[3]Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA

年份：2021

卷号：186

外文期刊名：ENVIRONMENTAL AND EXPERIMENTAL BOTANY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000644943400005)】；

基金：This work was supported by grants from the National Natural Science Foundation of China [31722012 and 31901304], Natural Science Foundation of Guangdong Province, China [2019A1515011595] and Fundamental Research Funds for the Central Non-profit Research Institution of CAF [CAFYBB2019QD001, and CAFYBB2020SY018].

语种：英文

外文关键词：ATAC-seq; Haustorium formation; Melatonin; N uptake and assimilation; RNA-seq

摘要：The facultative hemiparasite Santalum album can rely entirely on its own roots to obtain nutrients when growing without a host or can use its haustoria to partially rely on host plants for water and nutrients, especially nitrogen (N). However, how S. album responds to different N conditions has not been determined and no attempt has been made to improve N use by S. album. Our previous studies showed that melatonin, which is known to promote tolerance to abiotic stresses in plants, enhances S. album growth. Here, we investigated the mechanisms and key pathways by which melatonin affects N uptake and assimilation in S. album. We evaluated changes in the physiology, transcriptional profiles, and chromatin accessibility in S. album seedlings exposed to low N (LN) or high N (HN) with or without 1 ?M melatonin addition. Without melatonin addition, LN disturbed the homeostasis of N metabolism, reducing both N uptake and assimilation. Melatonin addition, in contrast, significantly increased NH4+ uptake under LN and increased NO3? uptake under HN. Melatonin also increased the activities of NR and GS enzymes under LN, resulting in accelerated N metabolism and enhanced haustorium development via a mechanism associated with auxin accumulation. Using global RNA-seq and ATAC-seq, we found that many genes were differentially expressed in response to melatonin addition. The upregulated genes were enriched in N metabolism and hormone signal transduction (auxin) pathways, suggesting that they have key roles in improving N use by S. album. These results strongly suggest that the application of melatonin may enhance S. album growth via accelerated N metabolism and haustorium formation.