文献类型：期刊文献

中文题名：云南松基因组微卫星序列特征分析

英文题名：Characteristic Analysis of Microsatellites of Pinus yunnanensis

作者：贺圆[1,2] 王兵益[2] 廖声熙[2] 崔凯[2]

第一作者：贺圆

机构：[1]西南林业大学;[2]中国林业科学研究院资源昆虫研究所

年份：2017

卷号：32

期号：3

起止页码：104-107

中文期刊名：西北林学院学报

外文期刊名：Journal of Northwest Forestry University

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2017_2018】；

基金：国家"十二五"科技支撑计划课题(2012BAD01B0203)

语种：中文

中文关键词：云南松;基因组;微卫星;重复类型

外文关键词：Pinus yunnanensis ; genome; microsatellite; repeat type

分类号：S791.257

摘要：为了更好地了解云南松基因组特征,分析了云南松基因组中微卫星序列的分布特征,采用FLAF-seq测序技术,共获得1 970条微卫星重复序列,利用MISA通过对Unigene序列的分析,鉴定出7种类型的SSR。7种微卫星序列中主要以单碱基重复类型为主,共1 104个,约占重复序列总数的56.04%,其次是双碱基和三碱基重复类型,分别占总数的27.66%和11.68%,四碱基重复类型数量30个,占重复序列总数的1.52%,五碱基、六碱基重复类型在总重复序列中占比最少,均为0.2%,混合微卫星占2.7%。在单碱基重复类型中占优势的重复单元是A/T碱基,双碱基重复类型中占优势的重复单元是(AT)_n/(TA)_n碱基,三、四、五碱基重复类型中,占优势的重复单元不明显,但几种碱基重复类型中,都包含着丰富的A和T。所得到的微卫星重复序列中以长度<20bp序列的微卫星数量最多,微卫星长度≥20bp的只占总数的10.81%。可为后续云南松遗传多样性的研究及SSR引物筛选等提供一定的理论依据。
In order to better understand the genomic features of Pinus yunnanensis, FLAF-seq sequencing technology was used to analyze the distribution and variation of SSR from genomic DNA of P. yunnanen- sis. Totally 1 970 microsatellite repeats were identified by using MlcroSAtellite identification tool. Through the analysis of unigene sequence, seven types of SSR were recognized. Among them, mono-nucleotide repeat types had the largest number （1 104）,accounting for 56.04% of the total repeating sequences,followed by di-nucleotide and tri-nucleotide repeat types, accounting for 27.66% and 11.68%, respectively. The number of tetra-nucleotide repeat types was 30 （1. 52%）, and penta-nucleotide and hexa-nucleotide repeat types were only accounting for 0.2 %. Compound SSR accounted for 2.7 %. Dominant type in mono-nucleotide re- peat types units was A/T, and di-nucleotide repeat types were （AT）n/（TA）n. The dominant repeat units in tri-nucleotide, tetra-nucleotide, penta-nucleotide, and hexa-nucleotide were not obvious. However, all of them contained a wealth of A and T. Among the obtained microsatellite repeat sequences, most of them were less than 20 bp. The microsatellite of more than or equal to 20 bp only occupied 10.81% of the total sequence. This study would provide a theoretical basis for further research of P. yunnanensis genetic diver- sity and SSR primers screening.