文献类型：期刊文献

英文题名：A C2-domain phospholipid-binding protein MdCAIP1 positively regulates salt and osmotic stress tolerance in apple

作者：Liu, Xiao-Juan[1,2] Dong, Yuan-Hua[1] Liu, Xin[1,3] You, Chun-Xiang[1] Hao, Yu-Jin[1]

第一作者：刘肖娟;Liu, Xiao-Juan

通信作者：Hao, YJ[1]

机构：[1]Shandong Agr Univ, Coll Hort Sci & Engn, MOA Key Lab Hort Crop Biol & Germplasm Innovat, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Beijing Forestry Univ, Coll Biol Sci & Technol, Natl Engn Lab Tree Breeding, 35 Qinghua East Rd, Beijing 100083, Peoples R China

年份：2019

卷号：138

期号：1

起止页码：29-39

外文期刊名：PLANT CELL TISSUE AND ORGAN CULTURE

收录：;EI(收录号：20191606791726);Scopus(收录号：2-s2.0-85064209830);WOS:【SCI-EXPANDED(收录号:WOS:000471312500003)】；

基金：This work was supported by grants from National Natural Science Foundation of China (31430074, U1706202), the Ministry of Agriculture of China (CARS-28), Shandong Province (SDAIT-06-03), and the Ministry of Education of China (IRT15R42).

语种：英文

外文关键词：MdCAIP1; Salt and osmotic stress; C2-domain; Homodimer; Apple

摘要：High salt restricts the growth and development of plants. A diverse range of genes are involved in the response to salt stress. Here we report that MdCAIP1 (C2-domain ABA Insensitive Protein1), a single C2-domain containing protein, has a conserved structure consisting of an eight-stranded anti-parallel -sandwich. MdCAIP1 was able to bind to phospholipids in a Ca2+-dependent manner and localized to the plasma membrane. qRT-PCR analysis showed that MdCAIP1 was induced by abiotic stresses including salt, osmotic, and drought stress, as well as the hormone JA. Transgenic apple calli and Arabidopsis over-expressing MdCAIP1 were more tolerant to salt and osmotic stress, and had higher proline content and lower MDA content under these stressful conditions. Furthermore, MdCAIP1 could homodimerize. These results indicate that MdCAIP1 is a Ca2+-dependent phospholipid-binding protein, and implicate it in tolerance to salt and osmotic stress. Key messageMdCAIP1, a Ca2+-dependent phospholipid-binding protein, could homodimerize and positively regulated salt and osmotic tolerance in plants.