文献类型：期刊文献

英文题名：Genome-wide identification, classification, and expression analysis of the phytocyanin gene family in Phalaenopsis equestris

作者：Xu, L.[1,2] Wang, X. J.[1] Wang, T.[1] Li, L. B.[1]

第一作者：Xu, L.

通信作者：Li, LB[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Key Lab Silviculture State Forestry Adm, State Key Lab Forest Genet & Tree Breeding, Beijing 100091, Peoples R China;[2]Hunan Agr Univ, Coll Hort & Landscape, Changsha 410128, Peoples R China

年份：2017

卷号：61

期号：3

起止页码：445-452

外文期刊名：BIOLOGIA PLANTARUM

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000405279000005)】；

基金：This work was supported by grants from the Ministry of Science and Technology (No. 2012BAD01B0702 and No. 2013AA102607).

语种：英文

外文关键词：arabinogalactan proteins; early nodulin-like proteins; plantacyanin-like proteins; stellacyanin-like proteins; uclacyanin-like proteins

摘要：Phytocyanins (PCs) are ancient blue copper-binding proteins in plants that bind to single type I copper atoms and function as electron transporters. PCs play an important role in plant development and stress resistance. Many PCs are considered to be chimeric arabinogalactan proteins (AGPs). Previously, 38, 62, and 84 PC genes were identified in Arabidopsis thaliana, Oryza sativa, and Brassica rapa, respectively. In this study, we identified 30 putative PC genes in the orchid Phalaenopsis equestris through comprehensive bioinformatics analysis. Based on phylogeny and motif constitution, the P. equestris phytocyanins (PePCs) were divided into five subclasses: 10 early nodulin-like proteins, 10 uclacyanin-like proteins, five stellacyanin-like proteins, four plantacyanin-like proteins, and one unknown protein. Structural and glycosylation predictions suggested that 16 PePCs were glycosylphosphatidylinositol-anchored proteins localized to the plasma membrane, 22 PePCs contain N-glycosylation sites, and 14 are chimeric AGPs. Phylogenetic analysis indicated that each subfamily was derived from a common ancestor before the divergence of monocot and dicot lineages and that the expansion of the PC subfamilies occurred after the divergence of orchids and Arabidopsis. The number of exons in PC genes was conserved. Expression analysis in four tissues revealed that nine PC genes were highly expressed in flowers, stems, and roots, suggesting that these genes play important roles in growth and development in P. equestris. The results of this study lay the foundation for further analysis of the functions of this gene family in plants.