文献类型：期刊文献

英文题名：Dual regulation of xylem formation by an auxin-mediated PaC3H17-PaMYB199 module in Populus

作者：Tang, Xianfeng[1] Wang, Dian[1] Liu, Yu[2] Lu, Mengzhu[3] Zhuang, Yamei[1,4] Xie, Zhi[4,5,6] Wang, Congpeng[2] Wang, Shumin[1] Kong, Yingzhen[7] Chai, Guohua[1,2] Zhou, Gongke[1]

第一作者：Tang, Xianfeng

通信作者：Chai, GH[1];Zhou, GK[1];Chai, GH[2]

机构：[1]Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China;[2]Qingdao Agr Univ, Coll Resources & Environm, Qingdao 266109, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[4]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[5]Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China;[6]Chinese Acad Sci, Ctr Excellence Mol Plant Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China;[7]Qingdao Agr Univ, Coll Agron, Qingdao 266109, Peoples R China

年份：2020

卷号：225

期号：4

起止页码：1545-1561

外文期刊名：NEW PHYTOLOGIST

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000510851200016)】；

基金：Financial support for this work was obtained from National Key Scientific Research Project of China (2016YFD0600104), National Key Program on Transgenic Research (2018ZX08020002), National Natural Science Foundation of China (31670606, 31570670, 31770315, and 31701068), Shandong Provincial Natural Science Foundation (ZR2017BC096 and ZR2017BC078), Youth Innovation Promotion Association of CAS (2017254), and Taishan Scholar Program of Shandong (to ZG). We are grateful to Professor Zhaojun Ding (Shandong University, China) for critical reading of the manuscript.

语种：英文

外文关键词：auxin; dual regulation; functional module; Populus; xylem formation

摘要：Wood (secondary xylem) formation in tree species is dependent on auxin-mediated vascular cambium activity in stems. However, the complex regulatory networks underlying xylem formation remain elusive. Xylem development in Populus was characterized based on microscopic observations of stem sections in transgenic plants. Transcriptomic, quantitative real-time PCR, chromatin immunoprecipitation PCR, and electrophoretic mobility shift assay analyses were conducted to identify target genes involved in xylem development. Yeast two-hybrid, pull-down, bimolecular fluorescence complementation, and co-immunoprecipitation assays were used to validate protein-protein interactions. PaC3H17 and its target PaMYB199 were found to be predominantly expressed in the vascular cambium and developing secondary xylem in Populus stems and play opposite roles in controlling cambial cell proliferation and secondary cell wall thickening through an overlapping pathway. Further, PaC3H17 interacts with PaMYB199 to form a complex, attenuating PaMYB199-driven suppression of its xylem targets. Exogenous auxin application enhances the dual control of the PaC3H17-PaMYB199 module during cambium division, thereby promoting secondary cell wall deposition. Dual regulation of xylem formation by an auxin-mediated PaC3H17-PaMYB199 module represents a novel regulatory mechanism in Populus, increasing our understanding of the regulatory networks involved in wood formation.