文献类型：期刊文献

英文题名：The bamboo delignification saturation point in alkaline hydrogen peroxide pretreatment and its association with enzymatic hydrolysis

作者：Huang, Chen[1] Zhan, Yunni[1] Cheng, Jinyuan[1] Wang, Jia[2] Meng, Xianzhi[3] Fang, Guigan[1] Ragauskas, Arthur J.[3,4,5]

第一作者：黄晨

通信作者：Fang, GG[1]

机构：[1]Chinese Acad Forestry, Inst Chem Ind Forest Prod, Jiangsu Prov Key Lab Biomass Energy & Mat, Nanjing 210042, Peoples R China;[2]Nanjing Forestry Univ, Coinnovat Ctr Efficient Proc & Utilizat Forest Re, Nanjing 210037, Peoples R China;[3]Univ Tennessee, Dept Chem & Biomol Engn, Knoxville, TN 37996 USA;[4]Univ Tennessee, Dept Forestry Wildlife & Fisheries, Ctr Renewable Carbon, Inst Agr, Knoxville, TN 37996 USA;[5]Oak Ridge Natl Lab, UTK ORNL Joint Inst Biol Sci, Biosci Div, POB 2009, Oak Ridge, TN 37831 USA

年份：2022

卷号：359

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20222612275982);Scopus(收录号：2-s2.0-85132745885);WOS:【SCI-EXPANDED(收录号:WOS:000848233900002)】；

基金：This research was supported by National Natural Science Foundation for Youth (32001273), Jiangsu Province Key Laboratory of Biomass Engineering and Materials (JSBEM-S-202004, JSBEM-S-202203), Natural Science Foundation of Jiangsu Province (BK2020789), and the National Natural Science Foundation of China (52006106). AJR efforts were supported by University of Tennessee, Knoxville.

语种：英文

外文关键词：Bamboo; Alkaline hydrogen peroxide pretreatment; Delignification saturation point; Enzymatic saccharification

摘要：A delignification saturation point (DSP) was observed for bamboo alkaline hydrogen peroxide pretreatment (AHP). Lignin removal was increased from 52.23% to similar to 70% when increasing H2O2 dosage from 0% to 2% at the optimum pH, but it cannot be further reinforced as increasing the H2O2. With partial lignin preserved, the glucan hydrolysis yield was found to have a ceiling of similar to 80%. This study indicated a strong association between enzymatic digestibility and lignin removal. Anatomical analysis by fluorescence microscope and confocal Raman microscope revealed that the undegradable lignin was mainly existing in the cell corner of sclerenchyma fibers, causing the DSP in the bamboo AHP. Finally, the residual lignin in pretreated bamboo was characterized with GPC, HSQC NMR, and P-31 NMR, which revealed the nature of DSP. This study could help to understand the lignin modification during the AHP and further contribute to the establishment of a chemical-saving biorefinery.