文献类型：期刊文献

英文题名：Binding properties of broad-specific monoclonal antibodies against three organophosphorus pesticides by a direct surface plasmon resonance immunosensor

作者：Jiao, Shasha[1] Liu, Pengyan[1] Liu, Ying[1] Zou, Rubing[1] Zhao, Ying[1] Liu, Yihua[1,2] Zhu, Guonian[1] Guo, Yirong[1]

第一作者：Jiao, Shasha

通信作者：Liu, YH[1];Guo, YR[1];Liu, YH[2]

机构：[1]Zhejiang Univ, Inst Pesticide & Environm Toxicol, Minist Agr, Key Lab Mol Biol Crop Pathogen & Insects, Hangzhou 310058, Zhejiang, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China

年份：2018

卷号：410

期号：28

起止页码：7263-7273

外文期刊名：ANALYTICAL AND BIOANALYTICAL CHEMISTRY

收录：;EI(收录号：20183805836875);Scopus(收录号：2-s2.0-85053417206);WOS:【SCI-EXPANDED(收录号:WOS:000447989800001)】；

基金：This study was financially supported by National Key R&D Program of China (2017YFF0210200 and 2016YFD0201302) and the Agricultural Project for Public Technology Research in Zhejiang Province (2016C32004).

语种：英文

外文关键词：OP pesticides; Heterologous icELISA; SPR immunosensor; Broad-specific mAb

摘要：In this study, heterologous indirect competitive enzyme-linked immunosorbent assay (icELISA) was introduced into the screening of hybridomas for the development of broad-specific monoclonal antibodies (mAbs) against organophosphorus (OP) pesticides. After immunization, two formats of icELISA based on the homologous hapten antigen and four heterologous hapten antigens were conducted for hybridoma screening. Two mAbs 2G6 and 7B2 with good recognition toward three OP pesticides (parathion, methyl-parathion, and fenitrothion) were produced. Results of the icELISA showed that the two mAbs exhibited high sensitivity against three OP pesticides, with IC50 ranging from 2.93 to 19.71ngmL(-1). Moreover, a non-competitive surface plasmon resonance (SPR) immunosensor was used for characterizing the binding properties of the mAbs to OP pesticides. After kinetic analysis, equilibrium dissociation constant (K-D) values of mAbs 2G6 and 7B2 were calculated as 1.45x10(-9)M and 4.26x10(-9)M for parathion, 6.75x10(-9)M and 4.17x10(-9)M for methyl-parathion, and 2.44x10(-8)M and 1.19x10(-8)M for fenitrothion, respectively. Whereas, both icELISA and SPR-based immunoassay indicated that the two mAbs could not recognize other five OP analogs. Since SPR-based immunoassay provides comprehensive information of two molecules directly interacting with each other, it is a valuable tool during the development and characterization of broad-specific mAbs.