文献类型：期刊文献

中文题名：春尺蠖新病毒株的鉴定及其对春尺蠖核型多角体病毒的增效作用

英文题名：Identification of New Virus Strain of Apocheima cinerarius and Its Synergistic Effect on A.cinerarius Nucleopolyhedrovirus

作者：王承锐[1,2] 李恩杰[3] 王少博[2] 王青华[2] 王玉珠[2] 张永安[2,3] 段立清[1]

第一作者：王承锐

机构：[1]内蒙古农业大学林学院,呼和浩特010019;[2]中国林业科学研究院森林生态环境与保护研究所/国家林业和草原局森林保护重点实验室,北京100091;[3]中国林业科学研究院华北林业实验中心,北京102300

年份：2020

卷号：36

期号：2

起止页码：196-202

中文期刊名：中国生物防治学报

外文期刊名：Chinese Journal of Biological Control

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：国家重点研发计划(2018YFD0600202)。

语种：中文

中文关键词：春尺蠖质型多角体病毒;春尺蠖核型多角体病毒;春尺蠖;增效作用

外文关键词：A.cinerarius cypovirus;A.cinerarius nucleopolyhedrovirus;Apocheima cinerarius;synergistic effect

分类号：S476.1

摘要：春尺蠖在我国危害严重,为了挖掘具有开发潜力和应用前景的生防因子,本文分离纯化一株能够感染春尺蠖幼虫的新病毒,生物活性测定了新病毒株的毒力及其对春尺蠖核型多角体病毒(ApciNPV)的增效作用。新病毒株的超微结构观察表明,该病毒多角体多为切面呈六边形的多面体,直径大小为0.32~1.20μm,病毒粒子呈近球状,具有质型多角体病毒的结构特征,命名为春尺蠖质型多角体病毒(ApciCPV)。生物活性测定结果显示,ApciCPV对春尺蠖3龄幼虫有很强的毒力,半致死浓度(LC50)为1.42×104 OBs/mL。当感染春尺蠖3龄幼虫时,ApciCPV可使ApciNPV半致死时间(LT50)缩短0.50~10.90 d,使ApciNPV对春尺蠖的最终致死率提高6.70%~43.33%。根据最终致死率、LT50以及ST三个值综合决定,当ApciNPV和ApciCPV配比浓度分别为1.0×106和1.0×104 OBs/mL时,对春尺蠖3龄幼虫的感染效率最高。新分离的ApciCPV病毒株是控制春尺蠖虫口密度的理想生物因子。
Apocheima cinerarius(Erschoff)is one of the major insect pests in China.A new virus strain that can infect the larvae of A.cinerarius was isolated and purified,and measured for its virulence and synergistic effect to A.cinerarius nucleopolyhedrovirus(ApciNPV).The ultrastructural observation of the new strain showed that the virus was mostly of a hexagonal polyhedron,with a diameter of 0.32~1.20μm,and with nearly spherical virion.With the structural features of a cypovirus,the virus was named as ApciCPV.Biological activity assays showed that ApciCPV was virulent against the 3rd instar larvae of A.cinerarius,with LC50 of 1.42×104 OBs/mL.Interestingly,ApciCPV showed a synergistic effect to ApciNPV.When the 3rd instar larvae of A.cinerarius were exposed to ApciCPV in combination with ApciNPV,LT50 of ApciNPV to the larvae was significantly shorten by 0.50―10.90 d,and the final lethal rate of ApciNPV increased by 6.70%―43.30%.Based on mortality,LT50 andST, the mixed solution of 1.0×106 OBs/mL ApciNPV and 1.0×104 OBs/mL ApciCPV, has the most virulenceagainst the 3rd instar larvae of A. cinerarius. These results show that the newly discovered viral strain, ApciCPV, isone of the ideal biological agents to control A. cinerarius.