文献类型：期刊文献

中文题名：运用AFLP技术分析筇竹种群遗传多样性

英文题名：Analysis of Populations Genetic Diversity of Qiongzhuea tumidinoda Using AFLP Markers

作者：茹广欣[1] 袁金玲[2] 张朵[1,2] 郭广平[2]

第一作者：茹广欣

机构：[1]河南农业大学林学院林学系;[2]中国林业科学研究院亚热带林业研究所

年份：2010

卷号：23

期号：6

起止页码：850-855

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：中国林科院亚热带林业研究所基本科研业务费项目(6807);"十一五"林业科技支撑计划专题(2006BAD19B0202);国家林业局重点项目(2006-64)

语种：中文

中文关键词：筇竹;遗传多样性;AFLP

外文关键词：Qiongzhuea tumidnoda; genetic diversity; AFLP

分类号：S795.9

摘要：利用AFLP分子标记技术对筇竹2个种群的遗传多样性进行了分析,筛选出10对多态性和清晰度较高的引物组合,共获得680个AFLP位点,其中多态性位点662个,平均多态性检出率为97.20%,并用PopGen32软件对AFLP多态性数据进行分析,结果表明,供试2个筇竹种群均具有丰富的遗传多样性,多态位点百分率分别为89.86%和91.95%,等位基因数分别为1.898 6和1.919 5,有效等位基因数分别为1.585 0和1.568 3,种群内遗传多样性为0.332 1,种群水平平均Ne’is遗传多样性为0.394 9,Shannon信息指数为0.575 4,基因流为2.900 9,遗传一致度为0.821 9,遗传距离平均值为0.196 1。并根据遗传距离进行了UPGMA聚类分析。
Amplified fragment length polymorphism（AFLP）was used to analyze the genetic diversity of 2 diferent populations of Qiongzhuea tumidnoda.Ten primer pairs with higher polymorphic were selected from EcoRI/MseI primers,Total of 680 loci of the Qiongzhuea tumidnoda genome were examined for molecular variation and 662 loci were polymorphic（97.20%）.PopGen32 data processing software gave out that the rate of polymorphism were 89.86% and 91.95%;the number of alleles were 1.898 6 and 1.919 5;the effective number of alleles were 1.585 0 and 1.568 3;the average genetic identities within 6 populations was 0.332 1;the average genetic identities within the 2 populations was 0.394 9;Shannon information index was 0.575 4.The genetic differentiation coefficient among the 2 populations was 0.148 8.The gene flow among the 2 populations was 2.861 0.The average genetic identity was 0.821 9 and the average genetic distance was 0.196 1.The 2 populations were divided according to the UPGMA cluster analysis.