文献类型：期刊文献

英文题名：Annulohypoxylon sp. FPYF3050 produces volatile organic compounds against the pine wood nematode, Bursaphelenchus xylophilus

作者：Li, Hongchang[1,2] Dou, Guiming[1] Gao, Mengge[3] Ren, Fei[4] Li, Ruhua[1] Zhang, Xingyao[3] Yan, Du-Hui[1,2]

第一作者：Li, Hongchang

通信作者：Yan, DH[1];Yan, DH[2]

机构：[1]Chinese Acad Forestry, Key Lab Forest Protect State Forestry Adm, Res Inst Forest Ecol Environm & Protect, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry New Technol, Beijing 100091, Peoples R China;[4]Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing 102300, Peoples R China

年份：2020

卷号：22

期号：3

起止页码：245-255

外文期刊名：NEMATOLOGY

收录：;Scopus(收录号：2-s2.0-85081618961);WOS:【SCI-EXPANDED(收录号:WOS:000519533200001)】；

基金：The authors thank Pengfei Wei, Jing Cui and Zhengkai Liu for their assistance in collecting the nematodes (eggs and J2); Wei Zhang in drawing and in vitro experiment; Yuqian Feng in statistical analysis. This project was financially supported by the National Key R&D Program of China (2017YFD0600100) and Fundamental Research Funds of CAF (CAFYBB2017MA010).

语种：英文

外文关键词：1,8-cineole; biological control; fungus; nematicidal activity; pine wilt disease

摘要：Natural volatiles released by the fungus. Annulohypoxylon sp. FPYF3050. were evaluated against the pine wood nematode (PWN), Bursaphelenchus xylophilus. Our results showed that volatile organic compounds (VOCs) caused 64.1 and 58.4% mortality of second-stage juveniles (J2) and mixed-stages (eggs, J2, third- and fourth-stage juveniles, and adults) of populations of PWN, but no inhibitive effects were detected on nematode eggs in the experiment. Analysis of the gases within the Petri plate by gas chromatography-mass spectrometry (GC-MS) showed a yield of an unique volatile with dominant 1,8-cineole in 77.4% relative area (RA) after 72 h treatment of nematodes with Annulohypoxylon sp. FPYF3050 and Botrytis cinerea. The commercial 1.8-cineole at concentrations of 2, 5, 10 and 15 mu l ml(-1) was applied to examine nematicidal activity. The results showed that 1.8-cineole had a 40-100% inhibition on the nematode eggs during 48 h treatment, more than 82.9% mortality of J2 after 24 h, 48 h and 96 h, and 18.7-91.9% mortality of the mixed-stage population, depending on the period after exposure. This result indicates that 1,8-cineole in the volatile gas emissions of Annulohypoxylon sp. FPYF3050 may play a crucial inhibitory effect on the pine wood nematode The nematicidal volatile gas from fungi may provide a useful biocontrol agent for controlling B. xylophilus.