文献类型：期刊文献

英文题名：Auxin-Induced SaARF4 Downregulates SaACO4 to Inhibit Lateral Root Formation in Sedum alfredii Hance

作者：Xu, Dong[1,2] Lu, Zhuchou[1,3] Qiao, Guirong[1,3] Qiu, Wenmin[1,3] Wu, Longhua[4] Han, Xiaojiao[1,3] Zhuo, Renying[1,3]

第一作者：Xu, Dong

通信作者：Han, XJ[1];Zhuo, RY[1];Han, XJ[2];Zhuo, RY[2]|[a00058eb5603bedf0c2e1]卓仁英;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Fac Forestry, Nanjing 210037, Peoples R China;[3]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Breeding Zhejiang Prov, Hangzhou 311400, Peoples R China;[4]Chinese Acad Sci, Natl Engn Lab Soil Pollut Control & Remediat Tech, Inst Soil Sci, Nanjing 210008, Peoples R China

年份：2021

卷号：22

期号：3

起止页码：1-15

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000615286200001)】；

基金：This work was supported by the Chinese Academy of Forestry foundation (CAFYBB 2019QB002), by the National Natural Science Foundation of China (No. 31872168), and by the Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding (No. 2016C020561).

语种：英文

外文关键词：SaARF4; SaACO4; ethylene; auxin; PINs; lateral root

摘要：Lateral root (LR) formation promotes plant resistance, whereas high-level ethylene induced by abiotic stress will inhibit LR emergence. Considering that local auxin accumulation is a precondition for LR generation, auxin-induced genes inhibiting ethylene synthesis may thus be important for LR development. Here, we found that auxin response factor 4 (SaARF4) in Sedum alfredii Hance could be induced by auxin. The overexpression of SaARF4 decreased the LR number and reduced the vessel diameters. Meanwhile, the auxin distribution mode was altered in the root tips and PIN expression was also decreased in the overexpressed lines compared with the wild-type (WT) plants. The overexpression of SaARF4 could reduce ethylene synthesis, and thus, the repression of ethylene production decreased the LR number of WT and reduced PIN expression in the roots. Furthermore, the quantitative real-time PCR, chromatin immunoprecipitation sequencing, yeast one-hybrid, and dual-luciferase assay results showed that SaARF4 could bind the promoter of 1-aminocyclopropane-1-carboxylate oxidase 4 (SaACO4), associated with ethylene biosynthesis, and could downregulate its expression. Therefore, we concluded that SaARF4 induced by auxin can inhibit ethylene biosynthesis by repressing SaACO4 expression, and this process may affect auxin transport to delay LR development.