文献类型：期刊文献

中文题名：Identification of novel cis-elements bound by BplMYB46 involved in abiotic stress responses and secondary wall deposition

英文题名：Identification of novel cis-elements bound by BplMYB46 involved in abiotic stress responses and secondary wall deposition

作者：Huiyan Guo[1] Liuqiang Wang[2] Chuanping Yang[1] Yiming Zhang[1] Chunrui Zhang[1] Chao Wang[1]

第一作者：Huiyan Guo

机构：[1]State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University;[2]State Key Laboratory of Tree Genetics and Breeding,Research Institute of Forestry,Chinese Academy of Forestry

年份：2018

卷号：60

期号：10

起止页码：1000-1014

中文期刊名：植物学报：英文版

收录：CSTPCD;;Scopus;CSCD:【CSCD2017_2018】；PubMed;

基金：supported by two grants from the National Natural Science Foundation of China (31470671 and 31700587)

语种：英文

中文关键词：生活;压力;墙;鉴定;目标基因;氨基丙酸;邮政部门;cis

分类号：Q943.2

摘要：Transcription factors(TFs)play vital roles in various biological processes by binding to cis-acting elements to control expressions of their target genes.The MYB TF BplMYB46,from Betula platyphylla,is involved in abiotic stress responses and secondary wall deposition.In the present study,we used a TF-centered yeast onehybrid technology(TF-centered Y1H)to identify the cisacting elements bound by BplMYB46.We screened a shortinsert random library and identified three cis-elements bound by BplMYB46:an E-box(CA(A/T/C)(A/G/C)TG)and two novel motifs,a TC-box(T(G/A)TCG(C/G))and a GT-box(A(G/T)T(A/C)GT(T/G)C).Chromatin immunoprecipitation(ChIP)and effector-reporter coexpression assays in Nicotiana tabacum confirmed binding of BplMYB46 to the TC-box,GT-box,and E-box motifs in the promoters of the phenylalanine ammonia lyase(PAL),peroxidase(POD),and superoxide dismutase(SOD)genes,which function in abiotic stress tolerance and secondary wall biosynthesis.This finding improves our understanding of potential regulatory mechanisms in the response to abiotic stress and secondary wall deposition of BplMYB46 in B.platyphylla.
Transcription factors （TFs） play vital roles in various biological processes by binding to cis-acting elements to control expressions of their target genes. The MYB TF BplMYB46, from Betula platyphylla, is involved in abiotic stress responses and secondary wall deposition. In the present study, we used a TF-centered yeast onehybrid technology （TF-centered YIH） to identify the cis- acting elements bound by BplMYB46. We screened a shortinsert random library and identified three cis-elements bound by BplMYB46： an E-box （CA（A/T/C）（A/G/C）TG） and two novel motifs, a TO-box （T（GIA）TCG（C/G）） and a GT-box （A（G/T）T（AIC）GT（T/G）C）. Chromatin immunoprecipitation （CHIP） and effector-reporter coexpression assays inNicotiana tabacum confirmed binding of BplMYB46 to the TC-box, GT-box, and E-box motifs in the promoters of the phenylalanine ammonia lyase （PAL）, peroxidase （POD）, and superoxide dismutase （SOD） genes, which function in abiotic stress tolerance and secondary wall biosynthesis. This finding improves our understanding of potential regulatory mechanisms in the response to abiotic stress and secondary wall deposition of BplMYB46 in B. platyphylla.