文献类型：期刊文献

英文题名：LC-MS method for detecting prostratin in plant extracts and identification of a high-yielding population of Euphorbia fischeriana

作者：Tang, Qi[7] Su, Zhiheng[6] Han, Zhentai[5] Ma, Xiaojun[7] Xu, Derong[4] Liang, Yanhai[3] Cao, Haibo[3] Wang, Xuesong[3] Qu, Xiaoying[3] Hoffman, Angela[1] Liu, Hongwei[4] Gu, Dizhou[2] Qiu, Deyou[4]

第一作者：Tang, Qi

通信作者：Hoffman, A[1]

机构：[1]Univ Portland, Dept Chem, Portland, OR 97203 USA;[2]Tonghua Normal Univ, Dept Biol, Tonghua 134002, Jilin, Peoples R China;[3]Daxinganling Forest Grp Co Ltd, Acad Agr & Forestry Sci, Jiagedaqi 165000, Peoples R China;[4]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Res Inst Forestry, Beijing 100091, Peoples R China;[5]Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Beijing 100091, Peoples R China;[6]Guangxi Med Univ, Sch Pharmaceut Sci, Nanning 530021, Peoples R China;[7]Chinese Acad Med Sci, Inst Med Plant Dev, Guangxi Branch Inst, Nanning 530023, Peoples R China

年份：2012

卷号：5

期号：1

起止页码：214-218

外文期刊名：PHYTOCHEMISTRY LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000300395900046)】；

基金：The authors are thankful for a grant from The Research Institute of Forestry (ZD200902) for financial support and wish to thank Prof. Guowei Qin (Shanghai Institute of Materia Medica) for the prostratin standard and NMR spectra. We thank Prof. Yanhai Liang for his assistance in the authentication of Euphorbia fischeriana, Euphorbia sp., E. esula, E. lucorum and Stellera chamaejasme.

语种：英文

外文关键词：Euphorbia fischeriana Steud.; Euphorbiaceae; LC-MS; Prostratin; 12-Deoxyphorbal-13-acetate

摘要：Prostratin, a tigliane phorbol ester with promise for the treatment of HIV, was identified and quantified in Euphorbia fischeriana root extracts obtained from several different sites in China. The greatest yield was recovered from root samples collected from Yakeshi, Inner Mongolia (63.54 mu g/g dry weight, or 0.00635% by mass). Prostratin was not detected in extracts of Euphorbia sp., E. esula, E. lucorum or Stellera chamaejasme. The presence of prostratin was verified by H-1 and C-13 NMR. Major fragmentation products were identified from ESI MSn spectra after HPLC separation, providing a reproducible fingerprint for unambiguously recognizing the compound in plant extracts. (C) 2011 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved.