文献类型：期刊文献

英文题名：Dynamics of physiological and miRNA changes after long-term proliferation in somatic embryogenesis of Picea balfouriana

作者：Li, Qingfen[1] Deng, Cheng[1] Zhu, Tianqing[2] Ling, Juanjuan[2] Zhang, Hanguo[3] Kong, Lisheng[4] Zhang, Shougong[2] Wang, Junhui[2] Chen, Xiaoyang[1]

第一作者：Li, Qingfen

通信作者：Chen, XY[1]

机构：[1]South China Agr Univ, Guangdong Key Lab Innovat Dev & Utilizat Forest P, State Key Lab Conservat & Utilizat Subtrop Agrobi, Coll Forestry & Landscape Architecture, Guangzhou 510642, Guangdong, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, Res Inst Forestry,State Forestry Adm, Beijing 100091, Peoples R China;[3]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Heilongjiang, Peoples R China;[4]Univ Victoria, Dept Biol, Ctr Forest Biol, Victoria, BC V8P 5C2, Canada

年份：2019

卷号：33

期号：2

起止页码：469-480

外文期刊名：TREES-STRUCTURE AND FUNCTION

收录：;EI(收录号：20190306398218);Scopus(收录号：2-s2.0-85060053044);WOS:【SCI-EXPANDED(收录号:WOS:000462316400013)】；

基金：The authors thank the Forestry Station of Xianggelila Country in Yunnan Province for cone sampling in the P. balfouriana seed orchard. This study was supported by grants from the General Financial Grant from China Postdoctoral Science Foundation (2017M622717) and the Fundamental Research Funds for the Central Nonprofit Research Institution of the Chinese Academy of Forestry (TGB2013011).

语种：英文

外文关键词：Embryogenic tissue; BA; Hormones; Antioxidant enzymes; Polyamines; miRNA

摘要：Key messageWe found that embryogenic tissues of Picea balfouriana adopt different physiological pathways for long-term proliferation with 6-benzylaminopurine treatment and identified the miRNAs closely associated with proliferation.AbstractThe long-term maintenance of somatic embryo production capacity in conifer embryogenic tissue (ET) is essential for the production of vigorous somatic seedlings. However, this ability is often lost after several months of proliferation in many conifer species including Picea balfouriana. Cytokinins are known to influence several important physiological processes during plant growth and development, including somatic embryogenesis (SE). In this study, we found that the 6-benzylaminopurine (BA) concentration influenced the yields of P. balfouriana somatic embryos and their germination response. Only ET of P. balfouriana proliferated on medium supplemented with 3.6 mu M BA produced somatic embryos that germinated into normal plants. Most hormone levels increased in ET after prolonged proliferation. Moreover, antioxidant enzyme activities and polyamine contents were also significantly changed after 8months of culture, which might be modulated by accumulated zeatin riboside (ZR). Finally, some selected microRNAs and their target genes were confirmed to be involved in the proliferation of ET of P. balfouriana and they also might be regulated by accumulated ZR. These findings may facilitate efforts to clarify basic physiological processes after the long-term proliferation stage of SE in conifers and delay the decreased production capacity of somatic embryos.