文献类型：期刊文献

中文题名：苹果叶片再生的改进及抗虫基因植株的获得

英文题名：Improvement of Excised Leaf Regeneration and Obtaining Bt Toxin Gene Transformated Plants of Apple Cultivars

作者：裴东[1] 田颖川[1] 刘群禄[1] 吴标[1] 奚声珂[1] 莽克强[1]

第一作者：裴东

机构：[1]中国林科院林业研究所,中国科学院微生物研究所,河北林学院

年份：1996

卷号：19

期号：4

起止页码：23-27

中文期刊名：河北农业大学学报

外文期刊名：JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI

收录：CSTPCD;;北大核心:【北大核心1992】；CSCD:【CSCD_E2011_2012】；

语种：中文

中文关键词：Bt基因;转化再生;苹果树;叶片

外文关键词：Leaf Regeneration; Btgene; Transformation; Apple

分类号：S661.1

摘要：用分别含有全部改造和部分改造的Ｂｔ基因植物表达载体ｐＢ４８．７和ｐＢ４８．６的土壤农杆菌转化苹果优良品种（红富士、早生富士、辽伏）的叶片外植体，经过对ＭＳ培养基成分调整，在Ｎｔ保持不变的条件下以（ＮＨ４）：ＳＯ４取代ＮＨ４ＮＯ３，使得转化后再生频率大增，现已获得２３２株转化再生植株，这些植株可以在合５０ｍｇ·Ｌ－１的卡那霉素培养基上生长，选出部分植株作ＰＣＲ检测，表明Ｂｔ基因已被导入这些苹果品种中。
The Leaf explants of three apple cultivars (Maluspumila Fuji, Yataka,Liao Fu)were genetically transformated using Agrobacterium tumefaciens contained totally and partly modified Bt toxin gene carried on plant expressing vectors pB48.7 andpB. By adjusting the component of MS regeneration medium, substituting(NH4)2SO4 for NH4NO3 in maintaining N level, the trans formated leaf regeneration washighly improved. 232 transformated plants have been obtained by selecting the shoots on the medium containing 50 mg/1 Kanamycin and analyzing of PCR. All these preliminarily showed the Bt toxin gene has been transfered into the apple cultivars.