文献类型：期刊文献

英文题名：Tissue-specific localization and dynamic changes of endogenous IAA during poplar leaf rhizogenesis revealed by in situ immunohistochemistry

作者：Dong, Ningguang[1,2] Pei, Dong[2] Yin, Weilun[1]

第一作者：Dong, Ningguang

通信作者：Yin, WL[1]

机构：[1]Beijing Forestry Univ, Sch Forestry, Beijing 100083, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Res Inst Forestry, Beijing 100091, Peoples R China

年份：2012

卷号：6

期号：2

起止页码：165-174

外文期刊名：PLANT BIOTECHNOLOGY REPORTS

收录：;Scopus(收录号：2-s2.0-84859895495);WOS:【SCI-EXPANDED(收录号:WOS:000303055200008)】；

基金：We sincerely thanked Dr Mingyong Chen (College of Veterinary Medicine, China Agricultural University, China) and Dr Zhixia Hou (School of Forestry, Beijing Forestry University, China) for their kind help with experimental methods and the equipment. This work was supported by the National Natural Science Foundation of China (Grant No. 31171933).

语种：英文

外文关键词：Rhizogenesis; Immunohistochemical localization; IAA; Leaf; Poplar; TIBA

摘要：Poplar 741 [Populus alba x (P. davidiana + P. simonii) x P. tomentosa] leaves were rooted within 8 days when cultured on 1/2 MS medium. The spatial distribution of endogenous indole-3-acetic acid (IAA) and its dynamic changes in the rhizogenesis were investigated, using an immunohistochemical approach. Anatomical analyses showed that the root primordia arose from vascular cambium cells in the basal regions of the petioles of the leaves. Before root induction, immunostaining patterns showed a basipetally decreasing gradient of IAA along the leaves. Three days after induction, the IAA immunostaining pattern observed along the leaves was high at both ends and low in the middle. And IAA in the basal regions of the petiole was distributed mainly in the vascular bundles. Localized application of 2,3,5-triiodobenzoic acid (TIBA) on laminas of the leaves delayed the accumulation of IAA in the vascular bundles of the basal regions of the petioles, but not in the mesophyll of the laminas. These data indicate that an accumulation of IAA in the vascular bundles of the basal regions of the petioles induces the occurrence of rhizogenesis of poplar leaves. And IAA accumulated in the vascular bundle of the basal region of the petiole results from its polar transportation from mesophyll of the laminas, rather than by in situ IAA generation.