文献类型：期刊文献

中文题名：紫花含笑TPS基因家族鉴定及与萜烯类物质代谢关系分析

英文题名：Identification of TPS Gene Family and Its Association with Terpenoid Metabolism in Michelia crassipes

作者：及浩楠[1,2] 肖宇光[3] 牛小云[1] 张志龙[2] 刘军[2] 姜景民[2] 刁姝[2]

第一作者：及浩楠

机构：[1]河北农业大学园林与旅游学院,河北保定071001;[2]中国林业科学研究院亚热带林业研究所,浙江杭州311400;[3]广西国有高峰林场,广西南宁530010

年份：2025

卷号：38

期号：3

起止页码：29-38

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：;北大核心:【北大核心2023】；

基金：浙江省农业(花卉新品种选育)新品种选育重大科技专项(2021C02071-3)。

语种：中文

中文关键词：紫花含笑;生物信息学;TPS基因家族;表达分析;挥发性萜类物质

外文关键词：Michelia crassipes;TPS genes family;Bioinformatics;Expression analysis;Volatile terpenoids

分类号：S722

摘要：[目的]探究紫花含笑花朵萜烯类物含量和TPS基因家族表达模式的相关性,为解析萜烯类物质合成途径的关键基因提供重要参考。[方法]基于紫花含笑无参转录组数据,采用生物信息学方法鉴定紫花含笑TPS基因家族成员,并对基因家族成员进行理化性质、保守基序及系统进化分析;利用实时荧光定量PCR技术揭示不同发育时期TPS基因表达模式,并结合顶空固相微萃取和气相色谱-质谱联用技术获取的不同发育阶段萜烯类物质含量,分析TPS基因表达和萜类物质含量的变化关系。[结果]本研究共鉴定到10个TPS成员,其编码氨基酸数量在511~987 aa,蛋白分子质量在57810.20~113803.45 Da。系统进化分析结果显示,紫花含笑TPS基因家族分为3个基因亚家族,TPS-e/f基因亚家族成员缺少Motif 1、Motif 3、Motif 5,TPS-b基因亚家族成员C41348.3缺少Motif 3,其余TPS基因含有6个Motif。C41348.6、C41348.3在时期Ⅴ显著性高表达,C31983.0和C40582.0在时期Ⅴ高表达。甜香型物质Α-罗勒烯、α-石竹烯、罗勒烯、卡地那二烯在时期IV含量上升,甜香型物质β-榄香烯、α-石竹烯、δ-榄香烯在时期V含量上升。对基因表达量变化与萜烯类物质含量变化进行相关性分析,紫花含笑TPS-b基因亚家族成员C41348.3和C41348.6均与单萜1,2-二[(E)-丙-1-烯基]环丁烷合成正相关。紫花含笑TPS-e/f基因亚家族中的C31983.0与3种甜果香型物质正相关。[结论]Α-罗勒烯、α-石竹烯、罗勒烯、卡地那二烯这几种甜果香型物质可能与紫花含笑时期IV花的果香形成有关。β-榄香烯、α-石竹烯、δ-榄香烯这几种甜果香型物质可能与紫花含笑时期V花的果香形成有关。C41348.6和C41348.3可能与成分1,2-二[(E)-丙-1-烯基]环丁烷合成有关。C31983.0可能参与调控紫花含笑甜果香型物质β-榄香烯、δ-榄香烯、α-石竹烯的形成。TPS基因家族可能在萜烯类物质的合成过程中发挥作用。本研究对紫花含笑TPS基因家族成员进行了深入挖掘,为未来探索紫花含笑花朵中重要萜类成分合成的关键基因功能提供理论基础。
[Objective]Terpenes are the substances with the highest number and content of volatile components in Michelia crassipes.This study aims to systematically identify TPS gene family members in M.crassipes,and elucidate their functional roles in terpenoid metabolism and floral aroma formation.[Methods]Based on the transcriptome sequencing data of M.crassipes,the TPS genes family of M.crassipes was identified by bioinformatics methods.The data obtained by qRT-PCR,headspace solid-phase microextraction and gas chromatography-mass spectrometry were used to analyze the relationship between TPS genes expression level and terpene metabolic content in different stages.[Results]A total of 10 TPS members were identified from the transcriptome data of M.crassipes.The molecular weight of the protein encoded by the TPS genes family members ranged from 57810.20 to 113803.45 Da,and the number of amino acids encoded ranged from 511 to 987.The results of phylogenetic analysis showed that the TPS gene family of M.crassipes was divided into three gene subfamilies.The TPS-e/f gene subfamily members lacked Motif 1,Motif 3 and Motif 5,and the TPS-a gene subfamily members contained 6 Motifs.The TPS-b gene subfamily member C41348.3 lacked Motif 3 and the remaining members contained 6 Motifs.TPS genes C41348.6 and C41348.3 were significantly highly expressed in stage V,while C31983.0 and C40582.0 also exhibited high expression at this stage.In terms of terpene content,A-ocimene,α-Caryophyllene,β-Ocimene,and cadinadiene were notably increased at stage IV.The content ofδ-elemene,α-Caryophyllene,β-Ocimene increased in stage V.The correlation between gene expression and terpene content was analyzed.The TPS-b gene subfamily members C41348.3 and C41348.6 were correlated with the synthesis of the 1,2-bis[(E)-prop-1-enyl]cyclobutane.C31983.0 in the TPS-e/f gene subfamily of M.crassipes was positively correlated with three types of sweet fruit aroma compounds.[Conclusion]The compounds A-ocimene,α-Caryophyllene,δ-elemene,and cadinadiene,may contribute to the fruity floral aroma characteristic of M.crassipes at stage IV,whileβ-elemene,α-caryophyllene,andδ-elemene are likely involved in aroma formation at stage V.TPS genes C41348.6 and C41348.3 may participate in the biosynthesis of 1,2-bis[(E)-prop-1-enyl]cyclobutane,whereas C31983.0 appears to regulate the formation of sweet fruity aroma-related compounds such asβ-elemene,α-caryophyllene,andδ-elemene.Overall,the TPS gene family plays a crucial role in terpene biosynthesis.This study provides an in-depth characterization of TPS gene family members in M.crassipes,offering a theoretical foundation for future research on the key genes involved in terpenoid biosynthesis in its petals.