文献类型：期刊文献

英文题名：Comparative metabolomic analysis highlights the involvement of sugars and glycerol in melatonin-mediated innate immunity against bacterial pathogen in Arabidopsis

作者：Qian, Yongqiang[1,2] Tan, Dun-Xian[3] Reiter, Russel J.[3] Shi, Haitao[1]

第一作者：Qian, Yongqiang;钱永强

通信作者：Shi, HT[1]

机构：[1]Hainan Univ, Coll Agr, Hainan Key Lab Sustainable Utilizat Trop Bioresou, Haikou 570228, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Univ Texas Hlth Sci Ctr San Antonio, Dept Cellular & Struct Biol, San Antonio, TX 78229 USA

年份：2015

卷号：5

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000363527600001)】；

基金：We thank Prof. Jianmin Zhou for sharing the bacterial strain Pst DC3000. This research was supported by the National Natural Science Foundation of China (No. 31570249) and the startup funding of Hainan University (No. kyqd1531) to Haitao Shi and by the Basic Research Fund of National Non-profit Research Institutions (No. CAFYBB2012043, RIF2014-03) to Yongqiang Qian.

语种：英文

摘要：Melatonin is an important secondary messenger in plant innate immunity against the bacterial pathogen Pseudomonas syringe pv. tomato (Pst) DC3000 in the salicylic acid (SA)-and nitric oxide (NO)-dependent pathway. However, the metabolic homeostasis in melatonin-mediated innate immunity is unknown. In this study, comparative metabolomic analysis found that the endogenous levels of both soluble sugars (fructose, glucose, melibose, sucrose, maltose, galatose, tagatofuranose and turanose) and glycerol were commonly increased after both melatonin treatment and Pst DC3000 infection in Arabidopsis. Further studies showed that exogenous pre-treatment with fructose, glucose, sucrose, or glycerol increased innate immunity against Pst DC3000 infection in wild type (Col-0) Arabidopsis plants, but largely alleviated their effects on the innate immunity in SA-deficient NahG plants and NO-deficient mutants. This indicated that SA and NO are also essential for sugars and glycerol-mediated disease resistance. Moreover, exogenous fructose, glucose, sucrose and glycerol pre-treatments remarkably increased endogenous NO level, but had no significant effect on the endogenous melatonin level. Taken together, this study highlights the involvement of sugars and glycerol in melatonin-mediated innate immunity against bacterial pathogen in SA and NO-dependent pathway in Arabidopsis.