文献类型：期刊文献

英文题名：Functional insights into JrWOX5: a WOX transcription factor regulating adventitious rooting and plant architecture in walnut

作者：Gao, Rui-xue[1] Zhang, Pu[1] Chang, Ying-ying[2] Song, Li[1] Song, Xiao-bo[1] Pei, Dong[1]

第一作者：Gao, Rui-xue

通信作者：Song, XB[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Silviculture Natl Forestry, Beijing 100091, Peoples R China;[2]Henan Normal Univ, Coll Life Sci, , Henan, Xinxiang 453007, Peoples R China

年份：2025

卷号：44

期号：12

外文期刊名：PLANT CELL REPORTS

收录：;Scopus(收录号：2-s2.0-105022106944);WOS:【SCI-EXPANDED(收录号:WOS:001615768700001)】；

基金：The research was funded by the Forestry and Grassland Science and Technology Innovation and Development Project (Grant number: 2025132018), the National Natural Science Foundation of China (Grant number: 32101479).

语种：英文

外文关键词：Walnut; Adventitious root formation; Ectopic expression; JrWOX5; Yeast two-hybrid assay

摘要：Key messageJrWOX5 promotes adventitious root formation and modulates plant architecture by interacting with key developmental regulators, providing novel insights into WOX-mediated organogenesis in woody plants.AbstractWUSCHEL-related homeobox (WOX) transcription factors, a plant-specific gene family, play essential roles in regulating plant development, including stem cell maintenance and organogenesis. Among the WOX genes identified in Juglans regia, JrWOX5 exhibited significantly elevated expression during AR formation, suggesting a potential regulatory role in this process. To investigate its function, we employed a combination of bioinformatics analysis, subcellular localization, heterologous overexpression, yeast two-hybrid (Y2H) assays, and bimolecular fluorescence complementation (BiFC). The JrWOX5 protein was localized to the nucleus. Ectopic expression of JrWOX5 in transgenic poplar markedly promoted AR formation and altered plant architecture, characterized by increased lateral branching and reduced plant height. To elucidate the molecular mechanism, protein-protein interaction (PPI) network analysis was conducted, and key candidate interactors were experimentally validated. JrWOX5 was found to interact with JrLBD16, a LOB domain-containing protein; JrLHW, a transcription factor; and JrCNR8, a regulator of cell proliferation. These findings indicate that JrWOX5 interacts with developmental regulators, which may jointly affect organogenesis and plant architectural patterning. This study aims to provide a new insight into WOX-associated organogenesis in woody plants.