文献类型：期刊文献

英文题名：Loss of the accessory chromosome converts a pathogenic tree-root fungus into a mutualistic endophyte

作者：Wei, Huanshen[1,2] Zhong, Zhenhui[3] Li, Zhongfeng[1,2] Zhang, Yuwei[1,2] Stukenbrock, Eva H.[4,5] Tang, Boping[6] Yang, Ningning[1,2] Baroncelli, Riccardo[7] Peng, Long[1,2] Liu, Zhuo[1,2] He, Xinghua[1,2] Yang, Yuzhan[1,2] Yuan, Zhilin[1,2]

第一作者：Wei, Huanshen

通信作者：Yuan, ZL[1];Yuan, ZL[2];Stukenbrock, EH[3];Stukenbrock, EH[4]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[3]Fujian Agr & Forestry Univ, State Key Lab Ecol Pest Control Fujian & Taiwan C, Fuzhou 350002, Peoples R China;[4]Univ Kiel, Environm Genom, D-24118 Kiel, Germany;[5]Max Planck Inst Evolutionary Biol, Max Planck Fellow Grp Environm Genom, D-24306 Plon, Germany;[6]Yancheng Teachers Univ, Sch Wetlands, Jiangsu Key Lab Bioresources Saline Soils, Yancheng 224002, Peoples R China;[7]Univ Bologna, Dept Agr & Food Sci DISTAL, I-40127 Bologna, Italy

年份：2024

卷号：5

期号：1

外文期刊名：PLANT COMMUNICATIONS

收录：;Scopus(收录号：2-s2.0-85171423146);WOS:【SCI-EXPANDED(收录号:WOS:001304310100001)】；

基金：This work was financially supported by the National Key Research and Development Program of China (2022YFD2201900) and the National Natural Science Foundation of China (no. 31722014).

语种：英文

外文关键词：dark septate endophytes; effectors; lifestyle transition; root-fungus symbioses

摘要：Some fungal accessory chromosomes (ACs) may contribute to virulence in plants. However, the mechanisms by which ACs determine specific traits associated with lifestyle transitions along a symbiotic continuum are not clear. Here we delineated the genetic divergence in two sympatric but considerably variable isolates (16B and 16W) of the poplar-associated fungus Stagonosporopsis rhizophilae. We identified a similar to 0.6-Mb horizontally acquired AC in 16W that resulted in a mildly parasitic lifestyle in plants. Complete deletion of the AC (Delta 16W) significantly altered the fungal phenotype. Specifically, Delta 16W was morphologically more similar to 16B, showed enhanced melanization, and established beneficial interactions with poplar plants, thereby acting as a dark septate endophyte. RNA sequencing (RNA-seq) analysis showed that AC loss induced the upregulation of genes related to root colonization and biosynthesis of indole acetic acid and melanin. We observed that the AC maintained a more open status of chromatin across the genome, indicating an impressive remodeling of cis-regulatory elements upon AC loss, which potentially enhanced symbiotic effectiveness. We demonstrated that the symbiotic capacities were non-host-specific through comparable experiments on Triticum- and Arabidopsis-fungus associations. Furthermore, the three isolates generated symbiotic interactions with a nonvascular liverwort. In summary, our study suggests that the AC is a suppressor of symbiosis and provides insights into the underlying mechanisms of mutualism with vascular plants in the absence of traits encoded by the AC. We speculate that AC-situated effectors and other potential secreted molecules may have evolved to specifically target vascular plants and promote mild virulence.