文献类型：期刊文献

英文题名：Decoding PHR-Orchestrated Stress Adaptation: A Genome-Wide Integrative Analysis of Transcriptional Regulation Under Abiotic Stress in Eucalyptus grandis

作者：Xu, Huiming[1,2] Xing, Yifan[2] Li, Guangyou[1] Wang, Xin[1,2] Zhou, Xu[2] Lu, Zhaohua[1] Ma, Liuyin[2] Yang, Deming[1]

第一作者：Xu, Huiming

通信作者：Yang, DM[1];Ma, LY[2]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Peoples R China;[2]Fujian Agr & Forestry Univ, Coll Forestry, Ctr Genom, Sch Future Technol,Fujian Prov Key Lab Haixia Appl, Fuzhou 350002, Peoples R China

年份：2025

卷号：26

期号：7

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;Scopus(收录号：2-s2.0-105002319421);WOS:【SCI-EXPANDED(收录号:WOS:001463926100001)】；

基金：This work was supported by the Fundamental Research Funds for the Central Non-profit Research Institution of CAF, grant number CAFYBB2022SY017, the National Key Research and Development Program of China during the 14th Five-year Plan Period, grant number 2022YFD2200203, the Forestry Peak Discipline Construction Project of Fujian Agriculture and Forestry University, grant number 72202200205.

语种：英文

外文关键词：PHR; transcription factor; gene expression; cold stress; salt stress; phosphate starvation; nitrogen starvation; boron deficiency; Eucalyptus grandis

摘要：The phosphate starvation response (PHR) transcription factor family play central regulatory roles in nutrient signaling, but its relationship with other abiotic stress remains elusive. In the woody plant Eucalyptus grandis, we characterized 12 EgPHRs, which were phylogenetically divided into three groups, with group I exhibiting conserved structural features (e.g., unique motif composition and exon number). Notably, a protein-protein interaction network analysis revealed that EgPHR had a species-specific protein-protein interaction network: EgPHR6 interacted with SPX proteins of multiple species, while Eucalyptus and poplar PHR uniquely bound to TRARAC-kinesin ATPase, suggesting functional differences between woody and herbaceous plants. A promoter sequence analysis revealed a regulatory network of 59 transcription factors (TFs, e.g., BPC, MYBs, ERFs and WUS), mainly associated with tissue differentiation, abiotic stress, and hormonal responses that regulated EgPHRs' expression. Transcriptomics and RT-qPCR gene expression analyses showed that all EgPHRs dynamically responded to phosphate (Pi) starvation, with the expression of EgPHR2 and EgPHR6 exhibiting sustained induction, and were also regulated by salt, cold, jasmonic acid, and boron deficiency. Strikingly, nitrogen starvation suppressed most EgPHRs, highlighting crosstalk between nutrient signaling pathways. These findings revealed the multifaceted regulatory role of EgPHRs in adaptation to abiotic stresses and provided insights into their unique evolutionary and functional characteristics in woody plants.