文献类型：期刊文献

中文题名：牡丹二氢黄酮醇4-还原酶基因PsDFR1的克隆及表达分析

英文题名：Cloning and Expression Analysis of Dihydroflavonol 4-Reductase Gene PsDFR1 from Tree Peony (Paeonia suffruticosa Andr.)

作者：周琳[1] 王雁[1] 任磊[1] 彭镇华[1]

第一作者：周琳

机构：[1]中国林业科学研究院林业研究所国家林业局林木培育重点实验室

年份：2011

卷号：47

期号：9

起止页码：885-892

中文期刊名：植物生理学报

外文期刊名：Plant Physiology Journal

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家"863"项目(2006AA100109);国家林业局"948"项目(2006-4-C07)

语种：中文

中文关键词：牡丹;二氢黄酮醇4-还原酶基因;克隆;表达

外文关键词：tree peony （Paeonia suffruticosa）; dihydroflavonol 4-reductase gene; cloning; expression

分类号：Q343.1

摘要：二氢黄酮醇4-还原酶(DFR)是花色素苷合成途径中的关键酶,在植物花色的形成过程中起重要作用。本研究以牡丹品种‘彩绘’为试材,采用RT-PCR和RACE方法从花瓣中获得了一个牡丹DFR基因cDNA全长,命名为PsDFR1,GenBank登录号为HQ283448。序列分析结果表明,PsDFR1全长1242bp,包含一个长为1095bp的开放阅读框,编码364个氨基酸。生物信息学分析显示该基因编码的蛋白具有典型的DFR蛋白功能结构域,包括多个保守的短链脱氢/还原酶家族的特征位点,属于NADB_Rossmann超家族。氨基酸序列比对和系统进化树分析表明,PsDFR1与葡萄、棉花、毛果杨、梨等植物的DFR相似性都在75%以上。实时荧光定量PCR分析表明,PsDFR1在花色素大量积累的花瓣中表达量最高,其次是萼片和雄蕊,再次是叶片,在心皮中表达量最低。
Dihydroflavonol 4-reductase （DFR） is a key enzyme in the anthocyanin biosynthesis pathway, and plays a critical role in flower pigmentation. In this study, a full-length cDNA sequence of DFR gene was obtained from petals of tree peony （Paeonia suffruticosa ‘Cai Hui’） using RT-PCR and RACE, and named PsDFR1 （GenBank accession No. HQ283448）. Sequence analysis indicated that PsDFR1 is 1 242 bp in full length and has an open reading frame of 1 095 bp encoding a deduced polypeptide of 364 amino acids. Bioinformatic analysis showed that PsDFR1 had the typical functional domains of DFR protein, containing most conserved characteristic sites in short-chain dehydrogenase/reductase and belonging to the NADB_Rossmann superfamily. Sequence alignment and phylogenetic analysis revealed that PsDFR1 shared more than 75% homology with DFR from grape （Vitis vinifera）, cotton （Gossypium hirsutum）, Populus trichocarpa and pear （Pyrus communis）. Quantitative RT-PCR analysis indicated that PsDFR1 showed the highest transcript abundance in anthocyanin-pigmented petals, moderate levels in sepals and stamens, low level in leaves and the lowest level in carpels.