文献类型：期刊文献

中文题名：杉木杂交育种中亲本选配的方法研究

英文题名：Methods for Parent Apolegamy of Chinese-fir in Cross Breeding

作者：何贵平[1,2] 齐明[1,2] 程亚平[3] 徐肇友[4] 罗修宝[5] 何必庭[4]

第一作者：何贵平

机构：[1]中国林业科学研究院亚热带林业研究所;[2]浙江省林木育种技术研究重点实验室;[3]浙江省庆元县庆元林场;[4]浙江省龙泉市林科院;[5]浙江省遂昌县林业技术推广总站

年份：2016

卷号：38

期号：4

起止页码：646-653

中文期刊名：江西农业大学学报

外文期刊名：Acta Agriculturae Universitatis Jiangxiensis

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：浙江省"十二五"竹木育种专项(2012C12908-11);国家"十二五"科技支撑项目(2012BAD01B0201)~~

语种：中文

中文关键词：杉木;亲本选配;等位酶;母本细胞质效应;杂种优势群法;预测模型法

外文关键词：Cunninghamia lanceolata Hook; parent apolegamy; allozyme; female cytoplasm effects; HGM; FMM;

分类号：S718.46

摘要：杂交育种是杉木遗传育种的主要途径。为合理进行杂交亲本选配,利用12个杂交组合和4个自交后代的杉木材积生长数据,通过采用等位酶技术和生理学方法,获得杉木亲本的基因型信息、光合速率和发芽种子的平均呼吸强度。然后研究亲本等位酶和生理性状差异性,分别采用杂种优势群法和预测模型法,来确定杉木杂交时亲本选配模式。杂种优势群法是先用聚类分析法对亲本群体进行类群划分,然后根据杉木细胞遗传学的研究结果,决定杉木杂交时亲本的选配模式:采用高光合速率(叶绿素含量)的亲本做母本,高呼吸速率的亲本做父本。预测模型法是从预测杉木杂交组合平均值表现大小的角度,提出亲本选配的方法:采用多元回归分析,来建立杂交组合子代的平均生长表现与亲本等位酶差异及生理指标间的预测模型,并用10年生的5个杂交组合的资料来验证预测模型的可靠性,结果发现多元回归预测模型是成功的。杂种优势群法与预测模型法的研究结果是相互依存的,两者配合使用效果更佳。研究结果在杉木杂交育种实践中具有实用价值。
Cross breeding is the main approach to genetic breeding of Chinese fir.In order to match cross parents reasonably,growth data of 12 cross combinations and 4 inbreeding combinations were used for study.The genotypes of the Chinese fir parents were obtained through the allozyme analysis.The photosynthetic rate of the parents of Chinese-fir at fast-growing period was measured by an indirect procedure. The average respiration rate of the parents was determined by infrared CO2 respirometer（ GHX-3051H）.On the basis of the study of the allozyme diversity of the parents,heterotic grouping method（ HGM） and forecasting model method（ FMM）were adopted respectively to study the parents＇ apolegamy in crossing breeding of Chinese-fir.HGM depended on the results of the cluster analysis of parents allozyme information,then it was combined with the research results of the Chinese fir cytogenetics,selecting the parent with higher photosynthetic rate as female; selecting the parent with higher respiratory rate as male.FMM was carried out on the parents＇ apolegamy based on forecasting the average performance of the cross combinations.Firstly,using allozyme information and physiological index relating to the volume growth,multiple regression models were established to predict the average performance of the cross combinations and then the parent to be used as female or male could be confirmed.The accuracy of these models was verified by the growth data of five 10-year-old cross combinations. Validation of the models reliability is feasible.The results of the two methods between HGM and FMM are mutually interdependent,it is more effective that two methods should be used together.Therefore the HGM and FMM have application values in the practice of cross breeding of Chinese fir.