文献类型：期刊文献

英文题名：Flavonoid 3 '-hydroxylase of Camellia nitidissima Chi. promotes the synthesis of polyphenols better than flavonoids

作者：Jiang, Lina[1,2,3] Fan, Zhengqi[1,3] Tong, Ran[1] Yin, Hengfu[1,2,3] Li, Jiyuan[1,3] Zhou, Xingwen[4]

第一作者：Jiang, Lina

通信作者：Li, JY[1];Li, JY[2];Zhou, XW[3]|[a00051cc706af4dd001ab]周兴文;

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, State Key Lab Tree Genet & Breeding, Hangzhou 311400, Peoples R China;[3]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Forest Genet & Breeding, Hangzhou 311400, Peoples R China;[4]Fujian Univ Technol, Coll Architecture & Urban Planning, Fuzhou 350117, Peoples R China

年份：2021

卷号：48

期号：5

起止页码：3903-3912

外文期刊名：MOLECULAR BIOLOGY REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000655941400001)】；

基金：This work was supported by the National Key R&D Program of China (2019YFD1001005), the special funds for basic scientific research expenses of public welfare research institutes of the Chinese academy of forestry (CAFYBB2017ZF001), The National key projects for international scientific and technological innovation cooperation among governments (2016YFE0126100), The National Natural Science Foundation of China (No. 31860228), the Key projects of Natural Science Foundation of the Guangxi Zhuang Autonomous Region (2018GXNSFDA281007), and Scientific Research Foundation of Yulin Normal University for high-level talents (G2018025).

语种：英文

外文关键词：Camellia nitidissima; F3 ' H; Expression patterns; Functional verification; Polyphenol synthesis

摘要：Camellia nitidissima Chi. is an ornamental plant of the genus Camellia L. Its flowers contain a lot of flavonoids and polyphenols. Flavonoid 3 '-hydroxylase (F3 ' H) plays an important role in the synthesis of flavonoids, polyphenols and anthocyanins. We used PCR amplification, quantitative PCR, High-performance liquid chromatography, subcellular localization, and agrobacterium-mediated leaf disk method to study the the function of CnF3 ' H. The full length of CnF3 ' H was 1859 bp (GenBank code: HQ290518.1), with an open reading frame of 1577 bp, and encoded 518 amino acid. A phylogenetic tree analysis showed that CnF3 ' H was closely related to Camellia sinensis L. and C. sinensis cultivar Zhonghuang. CnF3 ' H was expressed in flowers, leaves, fruits, sepals, petals and stamens of C. nitidissima, and during the flowering process the expression level in flower decreased initially and then increased. CnF3 ' H expression was significantly positive correlated with polyphenol contents in C. nitidissima. A CnF3 ' H-EGFP expression vector was constructed to do the subcellular localization, we found that CnF3 ' H was obviously localized in the nuclear envelope and cytomembrane. In transgenic tobacco flowers, the total polyphenol content and various polyphenol constituents were significantly increased with high CnF3 ' H expression level, while total flavonoid contents and some flavonol constituents were increased slightly. These findings suggest that CnF3 ' H promotes the synthesis of polyphenols better than flavonoids.