文献类型：期刊文献

英文题名：Transgenic poplar with the Zygophyllum xanthoxylon zinc finger protein transcription factor ZxZF gene shows improved drought resistance by regulating hormone signal

作者：Yao, Tongtong[1,2] Zhang, Hongjiao[1] Zhang, Weixi[2] Zhang, Zhe[1] Wang, Kexin[1] Zhao, Dongxue[3] Zhang, Hongrui[1] Sun, Nan[1] Zhang, Tanhang[1] Zhu, Wenxu[2] Ding, Changjun[2] Zhang, Huihui[1]

第一作者：Yao, Tongtong

通信作者：Zhang, HH[1];Ding, CJ[2]

机构：[1]Northeast Forestry Univ, Coll Life Sci, Key Lab Saline alkali Vegetat Ecol Restorat, Minist Educ, Harbin 150040, Heilongjiang, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry Ad, Beijing 100091, Peoples R China;[3]Shanghai Chongming Dist Changxing Town Agr Compreh, Shanghai 202150, Peoples R China

年份：2024

卷号：225

外文期刊名：ENVIRONMENTAL AND EXPERIMENTAL BOTANY

收录：;EI(收录号：20240152518);Scopus(收录号：2-s2.0-85196306362);WOS:【SCI-EXPANDED(收录号:WOS:001259458000001)】；

基金：This research was supported by the STI 2030-Major Projects (Grant No. 2023ZD0405603) , the National Major Project of GMO New Species Cultivation (Grant No. 2018ZX08020002) and the National Natural Science Foundation of China (Grant No. 32271751; 32000083) .

语种：英文

外文关键词：Zinc finger protein transcription factor; Poplar; Drought stress; Hormone signal transduction; Active oxygen metabolism

摘要：Drought stress has emerged as a primary factor constraining the growth, development, and yield of plants. The synthesis and signal transduction pathways of plant hormones play key roles in physiological adaptation to drought stress. The drought-resistance-associated C2H2 zinc finger protein transcription factor gene (ZxZF), which we previously identified, was transformed into Bofeng No. 1 poplar (Populus x euramericana c1. 'Bofeng1'). This study evaluated the mechanism of drought resistance improvement from the perspective of gene interaction networks between transcription factors and genes that regulate plant hormone signals. Transcriptome analysis shows that many differentially expressed genes (DEGs) between before and after drought and between wild-type (WT) and ZxZF-overexpression (OE) lines was significantly enriched in plant hormone signal transduction pathways in the KEGG pathway. Quantitative analysis of hormone content and expression of genes related to synthesis and signal transduction showed that abscisic acid (ABA) content and proline content in OE lines increased significantly after drought stress, which could not only enhance water retention ability of plants, but also avoid excessive amplification of ABA signals. Drought stress also increases the jasmonic acid (JA) content in OE lines, and JA can increase the content of antioxidant enzymes in OE lines through downstream signal transduction pathways, thus further eliminating ROS in plant leaves. In addition, as growth hormones, IAA and GA could be associated with ABA and JA through various downstream signal transduction pathways, and the interaction between hormones and hormone signal transduction pathways could jointly improve the drought resistance of ZxZF-OE lines.