文献类型：期刊文献

英文题名：Metabolic Profiling of Somatic Tissues from Monochamus alternatus (Coleoptera: Cerambycidae) Reveals Effects of Irradiation on Metabolism

作者：Qu, Liangjian[1] Wang, Lijuan[2] Wang, Qinghua[1] Wang, Yuzhu[1] Zhang, Yongan[1]

通信作者：Zhang, YA[1]

机构：[1]Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, State Forestry Adm, Key Lab Forest Protect, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2014

卷号：15

期号：6

起止页码：10806-10820

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000338639000095)】；

基金：This work was supported by the China "863" Project (No. 2012AAl01503) and the National Natural Science Foundation of China (No. 30972378). We greatly appreciate Jianjun Mou for providing help with larvae collection and the irradiation of adult beetles.

语种：英文

外文关键词：Monochamus alternatus; somatic cells; radiation; metabolomics

摘要：A high-level of sexual sterility is of importance for the sterile insect technique (SIT). However, the use of high-dose-intensity gamma radiation to induce sterility has negative impacts not only on reproductive cells but also on somatic cells. In this study, we investigated the metabolite differences in somatic tissues between non-irradiated, 20-Gy-irradiated, and 40-Gy-irradiated male Monochamus alternatus, an important vector of the pathogenic nematode, Bursaphelenchus xylophilus, which kills Asian pines. The results showed that metabolite levels changed moderately in the 20-Gy samples but were markedly altered in the 40-Gy samples compared with the non-irradiated samples. Twenty-six and 53 metabolites were disturbed by 20-Gy and 40-Gy radiation, respectively. Thirty-six metabolites were found to be markedly altered in the 40-Gy samples but were not changed significantly in the 20-Gy samples. The comprehensive metabolomic disorders induced by 40-Gy radiation dysregulated six metabolic pathways involved in the life process. The findings presented in this manuscript will contribute to our knowledge of the characteristic metabolic changes associated with gamma-radiation-induced damage to somatic cells and will allow for better exploration of the SIT for the control of this target pest.