文献类型：期刊文献

英文题名：Laccase Activities from Three White-rot Fungal Species Isolated from Their Native Habitat in North China Using Solid-State Fermentation with Lignocellulosic Biomass

作者：Liu, Wan-Xin[1] Zhao, Meng-Yao[1] Li, Mei-Xue[1] Li, Xue-Qing[1] Zhang, Tian-Xin[1] Chen, Xi[1] Yan, Xun-You[1,2,3] Bian, Lu-Sen[5] An, Qi[1,2,3] Li, Wen-Jing[1] Han, Mei-Ling[1,2,3,4]

第一作者：Liu, Wan-Xin

通信作者：Han, ML[1];Han, ML[2];Han, ML[3];Han, ML[4]

机构：[1]Langfang Normal Univ, Coll Life Sci, Langfang 065000, Hebei, Peoples R China;[2]Tech Innovat Ctr Utilizat Edible & Med Fungi Hebe, Langfang 065000, Hebei, Peoples R China;[3]Edible & Med Fungi Res & Dev Ctr Univ Coll Hebei, Langfang 065000, Hebei, Peoples R China;[4]Forestry Engn & Biodivers Conservat Technol Res &, Langfang 065000, Hebei, Peoples R China;[5]Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing 102300, Peoples R China

年份：2022

卷号：17

期号：1

起止页码：1533-1550

外文期刊名：BIORESOURCES

收录：;EI(收录号：20223712706832);Scopus(收录号：2-s2.0-85137260045);WOS:【SCI-EXPANDED(收录号:WOS:000760940600003)】；

基金：The authors gratefully acknowledge the financial support of the National Natural Science Foundation of China (Grant No. 31900009) , Special Project of Cultivating Scientific and Technological Innovation Ability of College and Middle School Students (Grant No. 2021H100404) , the Fundamental Research Funds for the Universities in Hebei Province (Grant No. JYQ202101) , and National Innovation and Entrepreneurship Training Program for College Students (Grant No. 202110100003) .

语种：英文

外文关键词：Laccase activity; Coriolopsis trogii; Trametes hirsuta; Phlebia acerina; Solid-state fermentation; Lignocellulosic biomass

摘要：Laccase has huge potential application in all aspects of biotechnology due to the ability of laccase to oxidize a wide range of phenolic and nonphenolic compounds. The future success of such applications requires large amounts of laccase with low costs. White-rot fungi are important groups of laccase production. In this study, three white-rot fungi, Phlebia 751, isolated from a native North China habitat, were identified by the method of molecular biology and preliminary screening of the ability of laccase-production by guaiacol selection medium. Then they were fermented on different lignocellulosic biomass. Three species showed consistency in preference of lignocellulosic biomass, and the presence of stalk of Sorghum bicolor was more suitable for secreting laccase. The capacity of laccase secretion from different species was significantly different. The capacity of secreting laccase of C. trogii Han 751 was superior to that of P. acerina Han 618 and T. hirsuta Han 726. The discovery of a new strain with superior capacity of secreting laccase and suitable lignocellulosic biomass were helpful for laying a foundation for the optimization of the fermentation conditions for the highest laccase production, the isolation and purification of laccase, and the industrial application of laccase.