文献类型：期刊文献

英文题名：Comparative proteomic analysis in Miscanthus sinensis exposed to antimony stress

作者：Xue, Liang[1,2] Ren, Huadong[1] Li, Sheng[1] Gao, Ming[1,2] Shi, Shengqing[2] Chang, Ermei[2] Wei, Yuan[2] Yao, Xiaohua[1] Jiang, Zeping[2] Liu, Jianfeng[2]

第一作者：Xue, Liang

通信作者：Liu, Jianfeng

机构：[1] Research Institute of Subtropical Forestry, Chinese Academy Forestry, Fuyang, Zhejiang, 311400, China; [2] State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China

年份：2015

卷号：201

起止页码：150-160

外文期刊名：Environmental Pollution

收录：EI(收录号：20151300685926);Scopus(收录号：2-s2.0-84925424140)

语种：英文

外文关键词：Metabolism - Plants (botany) - Signal transduction - Inductively coupled plasma - Antimony - Cell proliferation - Electrophoresis

摘要：Abstract To explore the molecular basis of Sb tolerance mechanism in plant, a comparative proteomic analysis of both roots and leaves in Miscanthus sinensis has been conducted in combination with physiological and biochemical analyses. M. sinensis seedlings were exposed to different doses of Sb, and both roots and leaves were collected after 3 days of treatment. Two-dimensional gel electrophoresis (2-DE) and image analyses found that 29 protein spots showed 1.5-fold change in abundance in leaves and 19 spots in roots, of which 31 were identified by MALDI-TOF-MS and MALDI-TOF-TOF-MS. Proteins involved in antioxidant defense and stress response generally increased their expression all over the Sb treatments. In addition, proteins relative to transcription, signal transduction, energy metabolism and cell division and cell structure showed a variable expression pattern over Sb concentrations. Overall these findings provide new insights into the probable survival mechanisms by which M. sinensis could be adapting to Sb phytotoxicity. ? 2015 Elsevier Ltd. All rights reserved.