文献类型：期刊文献

英文题名：Transcriptomic Analysis Reveals the Mechanism of Picea crassifolia Survival for Alpine Treeline Condition

作者：Shi, Zheng[1] Deng, Xiuxiu[1] Bai, Dengzhong[1] Lei, Jingpin[2] Li, Maihe[3] Zeng, Lixiong[1] Xiao, Wenfa[1]

通信作者：Xiao, WF[1]

机构：[1]Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Natl Forestry & Grassland Adm, Key Lab Forest Ecol & Environm, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[3]Swiss Fed Res Inst WSL, Forest Dynam, Zuercherstr 11, CH-8903 Birmensdorf, Switzerland

年份：2020

卷号：11

期号：2

外文期刊名：FORESTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000519236600105)】；

基金：This work was support by the National Natural Science Foundation of China (31300496) and the National Key Research and Development Program of the Ministry of Science and Technology of China (no. 2016YFD0600201).

语种：英文

外文关键词：transcriptome; treeline; elevation; carbon limitation; Picea crassifolia

摘要：The physiological mechanisms driving treeline formation succession captured the attention of ecologists many years ago, yet they are still not fully understood. In this study, physiological parameters (soluble sugars, starch, and nitrogen) were investigated in combination with transcriptomic analysis in the treeline tree species Picea crassifolia. The study was conducted in the middle of Qilian Mountain Reserves, Gansu Province, China, within the elevation range of 2500-3300 m. The results showed that the concentrations of non-structural carbohydrates decreased with increasing elevation in the current-year needles and current-year branches, as well as in the coarse and fine roots. RNA-Seq demonstrated that 483 genes were upregulated and 681 were downregulated in the comparison of 2900 and 2500 m (2900 vs. 2500), 770 were upregulated and 1006 were downregulated in 3300 vs. 2500, and 282 were upregulated and 295 were downregulated in 3300 vs. 2900. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the differentially expressed genes were highly enriched in photosynthesis-related processes, carbon fixation and metabolism, and nitrogen metabolism. Furthermore, almost all photosynthesis-related genes were downregulated, whereas many genes involved in cuticle lipids and flavonoid biosynthesis were upregulated, contributing to the survival of P. crassifolia under the treeline condition. Thus, our study provided not only molecular evidence for carbon limitation hypothesis in treeline formation, but also a better understanding of the molecular mechanisms of treeline tree survival under adverse conditions.