文献类型：期刊文献

英文题名：Callus induction and plant regeneration from leaves of peony

作者：Zhu, Xiangtao[1] Li, Xueqin[1] Ding, Wenjie[1] Jin, Songheng[1] Wang, Yan[2]

第一作者：Zhu, Xiangtao

通信作者：Jin, SH[1]

机构：[1]Zhejiang A&F Univ, Jiyang Coll, Zhuji 311800, Zhejiang, Peoples R China;[2]Chinese Acad Forestry, Key Lab Tree Breeding & Cultivat, State Forestry Adm Res Inst Forestry, Beijing 100091, Peoples R China

年份：2018

卷号：59

期号：4

起止页码：575-582

外文期刊名：HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY

收录：;Scopus(收录号：2-s2.0-85051139546);WOS:【SCI-EXPANDED(收录号:WOS:000441033000013)】；

基金：The work was partly supported by the Zhejiang Provincial Natural Science Foundation of China (LY16C160011), and the National Natural Science Foundation of China (Nos. 31170584 and 31200525).

语种：英文

外文关键词：Paeonia suffruticosa; Callus differentiation; Plant growth regulators; Root formation; Morphological analysis

摘要：Tree peony (Paeonia suffruticosa Andr.) is a valued ornamental plant. This study reports on peony callus induction, shoot organogenesis and plant regeneration using young peony leaves as explants. Various media containing diverse plant growth regulators were assessed for their potency in peony propagation. After exposure of dark-adapted leaf discs to 30 mu mol m(-2) s(-1) of light, inoculation in Murashige and Skoog (MS) + 0.2 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) + 0.2 mg L-1 a-naphthaleneacetic acid (NAA) + 3.0 mg L-1 thidiazuron (TDZ) medium resulted to the highest callus induction rate with values reaching up to 87.8%. We identified that MS + 0.2 mg L-1 NAA + 2.0 mg L-1 6-benzyladenine (6-BA) + 2.0 mg L-1 kinetin (KT), with a multiplication coefficient of 3.025, to be the optimal medium for further callus proliferation under light. Inoculation in MS + 2.0 mg L(-1)6-BA + 0.2 mg L-1 NAA + 0.3 mg L-1 TDZ medium allowed 22.22% of callus cultures to differentiate into adventitious shoots, whereas a similar rate of root formation was detected when 1/2 MS + 0.1 mg L-1 NAA + 0.05 mg L-1 IBA + 30 g L-1 sucrose medium was used. Our findings provide important information on peony regeneration and present a new method for peony tissue culture that will potentially facilitate mass propagation and genetic engineering of peony plants.