文献类型：期刊文献

英文题名：Dynamic expression of small RNA populations in larch (Larix leptolepis)

作者：Zhang, Junhong[1,2] Wu, Tao[1] Li, Long[3] Han, Suying[3] Li, Xinmin[1,4] Zhang, Shougong[1] Qi, Liwang[1]

第一作者：Zhang, Junhong

通信作者：Zhang, SG[1]

机构：[1]Chinese Acad Forestry, Cell Biol Lab, Res Inst Forestry, Beijing 100091, Peoples R China;[2]Zhejiang Agr & Forestry Univ, Nurturing Stn, State Key Lab Subtrop Silviculture, Hangzhou 311300, Zhejiang, Peoples R China;[3]Chinese Acad Forestry, Key Lab, Res Inst Forest Ecol & Protect, Beijing 100091, Peoples R China;[4]Univ Calif Los Angeles, Dept Pathol & Lab Med, Los Angeles, CA 90095 USA

年份：2013

卷号：237

期号：1

起止页码：89-101

外文期刊名：PLANTA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000313030400008)】；

基金：This work was supported by the National Natural Science Foundation of China (grant no. 30830086), the National Basic Research Program of China (grant no. 2009CB119106), and the National High Technology Research and Development Program of China (grant no. SQ2010AA1000687004).

语种：英文

外文关键词：Larix leptolepis; Small RNA; MicroRNA; Long sRNA; Trans-acting siRNA

摘要：Small RNAs (sRNAs) are emerging as essential regulators of biological processes. However, several studies have reported that gymnosperms do not express appreciable amounts of 24-nt sRNAs, and conifers in particular may have a unique sRNA-silencing signature. Here, we compared the sRNA transcriptomes of Japanese larch somatic embryos (SE) and seedlings. SE sRNAs exhibited a length bias toward 24 nt, while seedlings showed a bias toward a 21-nt length. We also confirmed that larch is capable of producing 24-nt sRNAs based on a polyacrylamide gel analysis. The sRNA expression patterns varied according to developmental stage, which might be associated with Dicer-like 3 and RNA-dependent RNA polymerase2 (RDR2) levels. Our data suggest that many MIR loci that produce canonical microRNAs (miRNAs, 20-22 nt) and long sRNAs (23-26 nt) have dual functions; the latter were preferentially produced in SE compared to seedlings. However, the ratio of miRNAs to total sRNAs in seedlings was higher than in SE, and most miRNAs were upregulated in seedlings. Trans-acting small interfering RNAs (ta-siRNAs) generated from TAS3 triggered by miR390 were identified, and levels of the three detected ta-siRNAs peaked in mature embryos, which was not consistent with the lowest RDR6 level. These findings indicate that larch, and possibly other gymnosperms, shares a common sRNA pathway with other land plants, and that the sRNA distribution pattern varies according to developmental stage, which may be attributable to the expression of sRNA pathway genes.