文献类型：期刊文献

中文题名：红树莓NF-YA基因家族的鉴定及表达分析

英文题名：Identification and expression analysis of NF-YA gene family in red raspberry(Rubus idaeus)

作者：青梦瑶[1] 李明[1] 李迎超[2] 贾秀洁[1] 范青[1] 张雪梅[3] 郑奕宸[1] 张瑞杰[1] 李寒[3] 顾玉红[1]

第一作者：青梦瑶

机构：[1]河北农业大学生命科学学院,河北保定071001;[2]中国林业科学研究院林业研究所,北京100091;[3]河北农业大学林学院,河北保定071001

年份：2025

卷号：61

期号：7

起止页码：973-982

中文期刊名：植物生理学报

外文期刊名：Plant Physiology Journal

收录：;北大核心:【北大核心2023】；

基金：河北省重点研发计划项目(20326338D)。

语种：中文

中文关键词：红树莓;NF-YA基因家族;生物信息学;表达分析

外文关键词：red raspberry(Rubus idaeus);NF-YA gene family;bioinformatics;expression analysis

分类号：S663.2

摘要：核转录因子家族NF-YA在植物的生长发育和抗逆中具有重要作用。从红树莓果实转录组数据库中鉴定出5个NF-YA基因家族成员,对其生物信息学和不同组织表达模式进行分析。通过转录组测序、生物信息学分析、qRT-PCR技术,对NF-YA基因家族成员进行了鉴定和表达模式研究。结果表明:NF-YA基因家族5个成员的蛋白长度在204~354 aa之间,分子质量在22415.66~38587.16 Da之间,均为碱性不稳定的亲水性蛋白,磷酸化位点占比为丝氨酸>苏氨酸>酪氨酸磷酸化位点,无跨膜螺旋区和信号肽,均定位于细胞核上,二级结构和三级结构中以无规则卷曲为主,α螺旋次之,均含有CBF保守结构域,除了RuNF-YA1和RuNF-YA5蛋白之间不互作外,其他蛋白质之间都两两互作;RuNF-YAs基因含有5~7个外显子,RuNFYA1~RuNF-YA5依次含有8、7、7、8和4个保守基序;系统进化分析表明RuNF-YAs基因与月季、森林草莓、凤仙花亲缘关系较近;启动子顺式作用元件分析显示,RuNF-YAs启动子区域含有参与脱落酸反应、分生组织表达的顺式作用元件等;qRT-PCR和转录组结果显示,RuNF-YA基因家族在红树莓中的表达具有组织特异性,RuNF-YA1、RuNF-YA2和RuNF-YA5均在嫩根中表达量最高;在果实发育过程中,RuNF-YA基因家族的表达量均为青果时期最高,随着发育成熟呈下调表达,且与转录组趋势一致。综上所述,RuNF-YA可能与脱落酸等激素响应机制及根和果实的生长发育有关。
NF-YA plays an important role in plant growth and stress resistance.In this study,five members of NF-YA gene family were screened from the fruit transcriptome database of red raspberry(Rubus idaeus),and their bioinformatics and tissue expression were analyzed.The NF-YA gene family members were identified and their expression patterns were studied by transcriptome sequencing,bioinformatics analysis and qRT-PCR.The results showed that totally 5 members of NF-YA gene family are basic unstable hydrophilic proteins with the length of 204–354 aa and the molecular weight of 22415.66–38587.16 Da,the ratio of phosphorylation sites was serine>threonine>tyrosine,there were no transmembrane helical region and signal peptide,which were located in the nucleus,all contain a CBF conserved domain,with the exception that RuNF-YA1 and RuNF-YA5 proteins do not interact with each other,and all other proteins interact in pairs.The RuNF-YAs gene contains 5–7 exons,RuNF-YA1–5 contained 8,7,7,8 and 4 conserved motifs.Phylogenetic analysis showed that RuNF-YAs gene was closely related to Rosa chinensis,Fragaria vesca and Potentilla anserina.Promoter cis-acting element analysis showed that RuNF-YAs promoter region contained cis-acting elements involved in abscisic acid reaction and expression of meristems.The expression of RuNF-YA gene family was tissue-specific and the expression of RuNF-YA1,RuNF-YA2 and RuNF-YA5 were the highest in the tender root,and the expression of RuNF-YA gene family were the highest in the green fruit stage during fruit development,it was down-regulated with maturation,and the trend was consistent with the transcriptome.In conclusion,RuNF-YA gene family might be related to the response mechanism of abscisic acid and other hormones and the growth and development of roots and fruits.