文献类型：期刊文献

英文题名：Mycorrhizal Compatibility and Germination-Promoting Activity of Tulasnella Species in Two Species of Orchid (Cymbidium mannii and Epidendrum radicans)

作者：Yang, Qianyu[1,2] Xu, Lijun[1] Xia, Wei[3] Liang, Lixiong[1] Bai, Xiao[1] Li, Lubin[1] Xu, Lu[3] Liu, Lei[1]

第一作者：Yang, Qianyu

通信作者：Liu, L[1];Xu, L[2]

机构：[1]Res Inst Forestry, Chinese Acad Forestry, State Key Lab Forest Genet & Tree Breeding, Key Lab Silviculture State Forestry Adm, Beijing 100091, Peoples R China;[2]Shenyang Agr Univ, Coll Forestry, Shenyang 110866, Liaoning, Peoples R China;[3]Hunan Agr Univ, Coll Hort, Hunan Midsubtrop Qual Plant Breeding & Utilizat, Changsha 410128, Hunan, Peoples R China

年份：2021

卷号：7

期号：11

外文期刊名：HORTICULTURAE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000726824400001)】；

基金：This work was funded by the National Natural Science Foundation of China, grant number No. 31800522, and the project was supported by the Scientific Research Fund of Hunan Provincial Education Department, grant number No. 18B101 and the Hunan Provincial Natural Science Foundation of China, grant number No. 2019JJ50232.

语种：英文

外文关键词：Cymbidium mannii; Epidendrum radicans; Tulasnella spp; mycorrhizal fungi; symbiosis

摘要：In nature, Orchidaceae seeds establish a relationship with orchid mycorrhizal fungi to obtain essential nutrients for germination. The orchids, Cymbidium mannii and Epidendrum radicans, have significant ornamental and economic value. We isolated and cultured mycorrhizal fungi from C. mannii, E. radicans, and C. goeringii roots. Three strains of fungi, Tulasnella calospora (Tca), T. asymmetrica (Tas), and T. bifrons (Tbi), were identified using ITS-rDNA sequencing. Their mycorrhizal compatibility, germination-promoting effects, and symbiosis with the seeds of C. mannii and E. radicans were studied in vitro using various concentrations of oatmeal agar (OA) medium. Tca exhibited significant seed-germination-promoting effects on C. mannii (92.1%) and E. radicans (84.7%) on 2.0 and 4.0 g/L OA, respectively. For Tbi and Tas, the highest germination percentages were observed on 4.0 g/L OA in E. radicans (73.60% and 76.49%, respectively). Seed germination in C. mannii was enhanced by high oatmeal concentrations (8.0 and 12.0 g/L) during symbiosis with Tas, whereas Tbi had no effect regardless of OA concentration. Tca exhibited high compatibility with C. mannii and E. radicans, and the oatmeal concentration of the medium affected this compatibility. The findings of this study will aid in the propagation of endangered orchid species for conservation and commercial purposes using mycorrhizal technology.