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表流湿地细菌群落结构特征  ( EI收录)   被引量:13

Characteristics of Bacterial Communities in Surface-flow Constructed Wetlands

文献类型:期刊文献

中文题名:表流湿地细菌群落结构特征

英文题名:Characteristics of Bacterial Communities in Surface-flow Constructed Wetlands

作者:魏佳明[1,2,3] 崔丽娟[1,2,3] 李伟[1,2,3] 雷茵茹[1,2,3] 于菁菁[1,2,3] 秦鹏[1,2,3] 穆泳林[1,2,3] 梁钊瑞[1,2,3]

第一作者:魏佳明

通信作者:Cui, Li-Juan

机构:[1]中国林业科学研究院湿地研究所;[2]湿地生态功能与恢复北京市重点实验室;[3]北京汉石桥湿地生态系统国家定位观测研究站

年份:2016

卷号:37

期号:11

起止页码:4357-4365

中文期刊名:环境科学

外文期刊名:Environmental Science

收录:CSTPCD;;EI(收录号:20164603016223);Scopus(收录号:2-s2.0-84994388407);北大核心:【北大核心2014】;CSCD:【CSCD2015_2016】;PubMed;

基金:中央级公益性科研院所基本科研业务费专项(CAFYBB2014QA029)

语种:中文

中文关键词:表流湿地;细菌群落;水环境;氮;高通量测序

外文关键词:surface-flow constructed wetlands; microbial community; water environment; nitrogen; high-throughput sequencing

分类号:X172

摘要:为探讨表流湿地细菌群落结构的空间分布以及细菌环境影响因子,采用高通量测序方法,对表流湿地沿水流方向10个不同处理单元细菌群落结构进行分析,并利用冗余分析对其与环境因子的关系进行了探究.研究表明:细菌群落多样性指数(香农-威纳指数)平均值为6.57,细菌群落主要属于Proteobacterice(38.97%)、Bacteroidetes(15.63%)等18个门类,丰度大于1%的共有22个属;沿程细菌多样性总体呈现先升高后降低的波动性变化,最终处理单元与最初处理单元的多样性均较其余各处理单元低;细菌丰度与pH、ORP、NH_4^+-N、NO_2^--N、TN均有相关性.
Employing high-throughput sequencing as the method,this study analyzed the relationship between the water environment and the microbial community structure in the surface-flow constructed wetland. The results showed that: the mean Shannon-Wiener index was 6. 57 and there were mainly 18 categories in the microbial community,including Proteobacterice( 38. 97%),Bacteroidetes( 15. 63%) etc. Of these,the total content of 22 genera was over 1%. The microbial biodiversity showed an increasing trend at the beginning and then turned to a decreasing trend in the flowing direction. The results also revealed that pH,ORP,NH4^+-N,NO2^--N and TN acted as important restricting factors for the microbial community.

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