文献类型：期刊文献

英文题名：Distinctive Endophytic Fungal Assemblage in Stems of Wild Rice (Oryza granulata) in China with Special Reference to Two Species of Muscodor (Xylariaceae)

作者：Yuan, Zhi-lin[1,2] Su, Zhen-zhu[1] Mao, Li-juan[3] Peng, Yang-qing[4] Yang, Guan-mei[1] Lin, Fu-cheng[1] Zhang, Chu-long[1]

第一作者：Yuan, Zhi-lin;袁志林

通信作者：Zhang, CL[1]

机构：[1]Zhejiang Univ, Inst Biotechnol, Minist Agr, Key Lab Mol Biol Crop Pathogens & Insects, Hangzhou 310029, Zhejiang, Peoples R China;[2]Chinese Acad Forestry, Inst Subtrop Forestry, Fuyang 311400, Zhejiang, Peoples R China;[3]Zhejiang Univ, Inst Agrobiol & Environm Sci 985, Hangzhou 310029, Zhejiang, Peoples R China;[4]Zhejiang Univ, Coll Agr & Biotechnol, Dept Appl Biol Sci, Hangzhou 310029, Zhejiang, Peoples R China

年份：2011

卷号：49

期号：1

起止页码：15-23

外文期刊名：JOURNAL OF MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000287944400003)】；

基金：This work was financially supported by National Natural Science Foundation of China (Grant No. 30600002 and 30970097) to Chu-long Zhang.

语种：英文

外文关键词：endophytic fungi; singletons; host specificity; volatile organic compounds; antibiosis

摘要：Ecological niches in the rhizosphere and phyllosphere of grasses capable of sustaining endophytes have been extensively studied. In contrast, little information regarding the identity and functions of endophytic fungi in stems is available. In this study, we investigated the taxonomic affinities, diversity, and host specificities of culturable endophytes in stems of wild rice (Oryza grantilata) in China. Seventy-four isolates were recovered. Low recovery rate (11.7%) indicated that there were relatively few sites for fungal infection. Identification using morphology, morphospecies sorting, and molecular techniques resulted in classification into 50 taxa, 36 of which were recovered only once. Nucleotide sequence similarity analysis indicated that 30% of the total taxa recovered were highly divergent from known species and thus may represent lineages new to science. Most of the taxa were classified as members of the classes Sordariomycetes or Dothideomycetes (mainly in Pleosporales). The presence of Arthrinium and Magnaporthaceae species, most often associated with poaceous plants, suggested a degree of host specificity. A polyphasic approach was employed to identify two Muscodor taxa based on (i) ITS and RPB2 phylogenies, (ii) volatile compounds produced, and (iii) an in vitro bioassay of antifungal activity. This to our knowledge is only the second report regarding the isolation of Muscodor spp. in China. Therefore, we hypothesize that wild plants represent a huge reservoir of unknown fungi. The prevalence, novelty, and species-specificity of unique isolates necessitate a reevaluation of their contribution to ecosystem function and fungal biodiversity.